19 Kinds of Respiratory Pathogen Nucleic Acid Detection Kit (Fluorescence PCR)

Short Description:

This kit is used for the combined qualitative detection of SARS-CoV-2, influenza A virus, influenza B virus, adenovirus, mycoplasma pneumoniae, chlamydia pneumoniae, respiratory syncytial virus and parainfluenza virus(Ⅰ, II, III, IV) in throat swabs and sputum samples, human metapneumovirus, haemophilus influenzae, streptococcus pneumoniae, klebsiella pneumoniae, staphylococcus aureus, pseudomonas aeruginosa, legionella pneumophila and acinetobacter baumannii. 


Product Detail

Product Tags

Product name

HWTS-RT069A-19 Kinds of Respiratory Pathogen Nucleic Acid Detection Kit  (Fluorescence PCR) 

Channel

Channel Name

hu19 Reaction Buffer A

hu19 Reaction Buffer B

hu19 Reaction Buffer  C

hu19 Reaction Buffer  D

hu19 Reaction Buffer E

hu19 Reaction Buffer F

FAM Channel

SARS-CoV-2

HADV

HPIV Ⅰ

CPN

SP

HI

VIC/HEX Channel

Internal Control

Internal Control

HPIV Ⅱ

Internal Control

Internal Control

Internal Control

CY5 Channel

IFV A

MP

HPIV Ⅲ

Leg

PA

KPN

ROX Channel

IFV B

RSV

HPIV Ⅳ

HMPV

SA

Aba

Technical Parameters

Storage

≤-18℃ In dark  

Shelf-life

12 months

Specimen Type

Oropharyngeal swab samples, Sputum swab samples

CV

≤5.0%

Ct

≤40

LoD

300 Copies/mL

Specificity

the cross-reactivity study shows that there is no cross-reactivity between this kit and rhinovirus A, B, C, enterovirus A, B, C, D, human metapneumovirus, epstein-barr virus, measles virus, human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella-band herpes zoster virus, bordetella pertussis, streptococcus pyogenes, mycobacterium tuberculosis, aspergillus fumigatus, candida albicans, candida glabrata, pneumocystis jirovecii, cryptococcus neoformans and human genomic nucleic acid.

Applicable Instruments:

Applied Biosystems 7500 Real-Time PCR System

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd. 


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