28 Types of High-risk Human Papilloma Virus (16/18 Typing) Nucleic Acid
Product name
HWTS-CC006A-28 Types of High-risk Human Papilloma Virus (16/18 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Cervical cancer is one of the most common malignant tumors of the female reproductive tract. Studies have shown that HPV persistent infections and multiple infections are one of the main causes of cervical cancer. Currently, the recognized effective treatments are still lack for cervical cancer caused by HPV, so early discovery and prevention of cervical infection caused by HPV is the key to preventing cervical cancer. It is of great significance to establish a simple, specific and rapid etiology diagnostic test for the clinical diagnosis and treatment of cervical cancer.
Channel
| Reaction Mix | Channel | Type |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Internal Control | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Internal Control |
Technical Parameters
| Storage | Liquid: ≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | cervical exfoliated cell |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | 300Copies/mL |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1.
Recommended extraction reagents: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48), and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Option 2.
Recommended extraction reagents: Nucleic Acid Extraction or Purification Kit (YDP315) by Tiangen Biotech (Beijing) Co.,Ltd.
