4 Kinds of Respiratory Viruses Nucleic Acid Detection Kit (Fluorescence PCR)

Short Description:

This kit is used for the qualitative detection of SARS-CoV-2, influenza A virus, influenza B virus and respiratory syncytial virus nucleic acids in human oropharyngeal swab samples.


Product Detail

Product Tags

Product name

HWTS-RT075-Freeze-dried 4 Kinds of Respiratory Viruses Nucleic Acid Detection Kit (Fluorescence PCR)

Epidemiology

Corona Virus Disease 2019, referred to as "COVID-19", refers to the pneumonia caused by SARS-CoV-2 infection. SARS-CoV-2 is a coronavirus belonging to the β genus. COVID-19 is an acute respiratory infectious disease, and the population is generally susceptible. At present, the source of infection is mainly patients infected by SARS-CoV-2, and asymptomatic infected persons may also become the source of infection. Based on the current epidemiological investigation, the incubation period is 1-14 days, mostly 3-7 days. Fever, dry cough and fatigue are the main manifestations. A few patients had symptoms such as nasal congestion, runny nose, sore throat, myalgia and diarrhea, etc.

Influenza, commonly known as "flu", is an acute respiratory infectious disease caused by influenza virus. It is highly infectious. It is mainly transmitted by coughing and sneezing. It usually breaks out in spring and winter. Influenza viruses are divided into influenza A (IFV A), influenza B (IFV B), and Influenza C (IFV C) three types, all belong to sticky virus, cause human disease mainly for influenza A and B viruses, it is a single-stranded, segmented RNA virus. Influenza A virus is an acute respiratory infection, including H1N1, H3N2 and other subtypes, which are prone to mutation and outbreak worldwide. "Shift" refers to the mutation of influenza A virus, resulting in the emergence of a new virus "subtype". Influenza B viruses are divided into two lineages, Yamagata and Victoria. Influenza B virus only has antigenic drift, and it evades the human immune system's surveillance and elimination through its mutation. However, the evolution speed of influenza B virus is slower than that of human influenza A virus. Influenza B virus can also cause human respiratory infections and lead to epidemics.

Respiratory syncytial virus (RSV) is an RNA virus, belonging to the paramyxoviridae family. It is transmitted by air droplets and close contact and is the main pathogen of lower respiratory tract infection in infants. Infants infected with RSV may develop severe bronchiolitis and pneumonia, which are related to asthma in children. Infants have severe symptoms, including high fever, rhinitis, pharyngitis and laryngitis, and then bronchiolitis and pneumonia. A few sick children can be complicated with otitis media, pleurisy and myocarditis, etc. Upper respiratory tract infection is the main symptom of infection in adults and older children.

Channel

FAM SARS-CoV-2
VIC(HEX) RSV
CY5 IFV A
ROX IFV B
NED

Internal Control

Technical Parameters

Storage 2-8°C
Shelf-life 12 months
Specimen Type Oropharyngeal swab
Ct ≤38
LoD SARS-CoV-2: 150Copies/mL

Influenza A virus/Influenza B virus/Respiratory syncytial virus: 300Copies/mL

Specificity There is no cross-reactivity with human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s. pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci and newborn cryptococcus and human genomic nucleic acid.
Applicable Instruments Applied Biosystems 7500 Real-Time PCR System, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd. 


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