4 Kinds of Respiratory Viruses
Product name
HWTS-RT099- 4 Kinds of Respiratory Viruses Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Corona Virus Disease 2019, referred to as "COVID-19", refers to the pneumonia caused by 2019-nCoV infection. 2019-nCoV is a coronavirus belonging to the β genus. COVID-19 is an acute respiratory infectious disease, and the population is generally susceptible. At present, the source of infection is mainly patients infected by 2019-nCoV, and asymptomatic infected persons may also become the source of infection. Based on the current epidemiological investigation, the incubation period is 1-14 days, mostly 3-7 days. Fever, dry cough and fatigue are the main manifestations. A few patients had symptoms such as nasal congestion, runny nose, sore throat, myalgia and diarrhea, etc.
Channel
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Internal Control |
Technical Parameters
| Storage |
-18℃ |
| Shelf-life | 9 months |
| Specimen Type | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Copies/mLInfluenza A virus/Influenza B virus/Respiratory syncytial virus: 500Copies/mL |
| Specificity | a) Cross-reactivity results show that there is no cross reaction between the kit and human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci and newborn cryptococcus and human genomic nucleic acid. b) Anti-interference ability: select mucin (60mg/mL), 10% (v/v) of blood and phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (including preservatives) (20 mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir(500mg/mL), ritonavir(60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) and tobramycin (0.6mg/mL) for interference test, and the results show that interfering substances with concentrations mentioned above have no interference reaction to the test results of pathogens. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems |
Work Flow
Option 1.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) produced by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The extracted sample volume is 200μL, and the recommended elution volume is 80μL.
Option 2.
QIAamp Viral RNA Mini Kit (52904) produced by QIAGEN or Nucleic Acid Extraction or Purification Kit (YDP315-R) produced by Tiangen Biotech (Beijing) Co., Ltd. The extracted sample volume is 140μL, and the recommended elution volume is 60μL.
