Adenovirus Universal
Product name
HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Human adenovirus (HAdV) belongs to the genus Mammalian adenovirus, which is a double-stranded DNA virus without envelope. Adenoviruses that have been found so far include 7 subgroups (A-G) and 67 types, of which 55 serotypes are pathogenic to humans. Among them, could leading to the respiratory tract infections are mainly group B (Types 3, 7, 11, 14, 16, 21, 50, 55), Group C (Types 1, 2, 5, 6, 57) and Group E (Type 4), and could leading to intestinal diarrhea infection is Group F (Types 40 and 41)[1-8]. Different types have different clinical symptoms, but mainly respiratory tract infections. Respiratory diseases caused by respiratory tract infections of human body account for 5%~15% of global respiratory diseases, and 5%-7% of global childhood respiratory diseases[9]. Adenovirus is endemic in a wide range of areas and can be infected all year round, especially in crowded areas, which are prone to local outbreaks, mainly in schools and military camps.
Channel
| FAM | adenovirus universal nucleic acid |
| ROX |
Internal Control |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Nasopharyngeal swab，Throat swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300Copies/mL |
| Specificity | a) Test the standardized company negative references by the kit, and the test result meets the requirements.
b) Use this kit to detect and there is no cross-reactivity with other respiratory pathogens (such as Influenza A virus, Influenza B virus, Respiratory syncytial virus, Parainfluenza virus, Rhinovirus, Human metapneumovirus, etc.) or bacteria (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, etc.). |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR Systems, BioRad CFX Opus 96 Real-Time PCR Systems |
Work Flow
(1) Recommended extraction reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). The extraction should be performed according to the instructions. The extracted sample is the patients’ nasopharyngeal swab or throat swab samples collected on site. Add the samples into the sample release reagent by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex to mix well, place at room temperature for 5 minutes, take out and then invert and mix well to obtain the DNA of each sample.
(2) Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). the operation should be performed in strict accordance with the instructions. The extracted sample volume is 200μL, and the recommended elution volume is 80μL.
(3) Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent (YDP315) by Tiangen Biotech (Beijing) Co.,Ltd., the operation should be performed in strict accordance with the instructions. The extracted sample volume is 200μL, and the recommended elution volume is 80μL.
