Borrelia Burgdorferi Nucleic Acid
Product name
HWTS-OT068 Borrelia Burgdorferi Nucleic Acid Detection Kit(Fluorescence PCR)
Certificate
CE
Epidemiology
Lyme disease is caused by infection with Borrelia burgdorferi and is mainly transmitted between animal hosts, between host animals and humans by hard ticks. The pathogen Borrelia burgdorferi can cause human Erythema chronicum migrans, as well as diseases involving multiple systems such as heart, nerve, and joint, etc., and the clinical manifestations are diverse. According to the developing of diseases course, it can be divided into early localized infection, intermediate disseminated infection and late persistent infection, which are serious harm to the health of the population. Therefore, in the clinical diagnosis of Borrelia burgdorferi, it is of great significance to establish a simple, specific and rapid method for etiological diagnosis of Borrelia burgdorferi.
Channel
| FAM | DNA of Borrelia burgdorferi |
| VIC/HEX |
Internal Control |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Whole blood sample |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Copies/mL |
| Applicable Instruments | ABI 7500 Real-Time PCR Systems
ABI 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1.
QIAamp DNA Blood Midi Kit by Qiagen (51185). It should be extracted in strict accordance to the instruction, and the recommended elution volume is 100μL.
Option 2.
Blood Genomic DNA Extraction kit (DP318, No.: Jingchang Device Record 20210062) produced by Tiangen Biochemical Technology (Beijing) Co., Ltd.. It should be extracted in strict accordance to the instruction, and the recommended elution volume is 100μL.
Option 3.
Wizard® Genomic DNA Purification Kit (A1120) by Promega. It should be extracted in strict accordance to the instruction, and the recommended elution volume is 100μL.
