Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

Short Description:

This kit is used for qualitative typing detection of denguevirus (DENV) nucleic acid in suspected patient’s serum sample to help diagnose patients with Dengue fever.


Product Detail

Product Tags

Product name

HWTS-FE034-Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)
HWTS-FE004-Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

Certificate

CE

Epidemiology

Dengue fever (DF), which is induced by denguevirus (DENV) infection, is one of the most epidemic arbovirus infectious diseases. DENV belongs to flavivirus under flaviviridae, and can be classified into 4 serotypes according to surface antigen. Its transmission medium includes Aedes aegypti and Aedes albopictus, prevalent mainly in tropical and subtropical areas.

Clinical manifestations of DENV infection mainly include headache, fever, weakness, enlargement of lymph node, leukopenia and etc., and bleeding, shock, hepatic injury or even death in severe cases. In recent years, climate change, urbanization, quick development of tourism and other factors have provided more rapid and convenient conditions for transmitting and spreading of DF, leading to constant expansion of epidemic area of DF.

Channel

FAM Dengue Virus I
VIC(HEX) Dengue Virus II
ROX Dengue Virus III
CY5 Dengue Virus IV

PCR Amplification Conditions Setting

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 30mins No
2 1 cycle 95℃ 15mins No
3 40 cycles 95℃ 15s No
60℃ 45s Yes

Technical Parameters

Storage Liquid: ≤-18℃ in dark; lyophilization:≤30℃ in dark
Shelf-life 12 months
Specimen Type Fresh Serum
Ct ≤38
CV ≤5.0%
LoD 500 Copies/mL
Specificity Perform cross reaction tests of Japanese encephalitis virus, Forest encephalitis virus, severe fever with thrombocytopenia syndrome, Xinjiang hemorrhagic fever, hantaan virus, hepatitis C virus, influenza A virus, influenza B virus and etc. No cross reaction is detected
Applicable Instruments It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems
ABI 7500 Real-Time PCR Systems
ABI 7500 Fast Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems
LightCycler®480 Real-Time PCR Systems
LineGene 9600 Plus Real-Time PCR Detection Systems
MA-6000 Real-Time Quantitative Thermal Cycler
BioRad CFX96 Real-Time PCR System
BioRad CFX Opus 96 Real-Time PCR System

Total PCR Solution

Dengue Virus I II III IV Nucleic Acid Detection Kit6

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