SARS-CoV-2 Spike RBD Antibody

Short Description:

Enzyme-Linked Immunosorbent Assay for detecting SARS-CoV-2 Spike RBD Antibody was intended to detect the valence of Antibody of SARS-CoV-2 Spike RBD Antigen in serum/plasma from population inoculated by SARS-CoV-2 vaccine.


Product Detail

Product Tags

Product name

HWTS-RT055A-Enzyme-Linked Immunosorbent Assay for detecting SARS-CoV-2 Spike RBD Antibody

Certificate

CE

Epidemiology

Coronavirus Disease 2019 (COVID-19) is a pneumonia caused by infection with a new coronavirus named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-CoV-2 was a strain in beta-CoV virus capsulated particulates in round or elliptical shape with diameters about 60nm-140nm. COVID-19 is an acute respiratory infectious disease, and the population is generally susceptible. Currently known infection source of COVID-19 are infected COVID-19 cases and asymptomatic carrier of SARS-CoV-2. Population inoculated by SARS-CoV-2 vaccine can generate spike RBD antibody or S antibody of SARS-CoV-2 detectable in serum and plasma, which can be as indicator to evaluate the efficiency of inoculating SARS-CoV-2 vaccine.

Technical Parameters

Storage

2-8℃

Shelf-life

12 months

Specimen Type

human serum, plasma, samples with anticoagulant of EDTA, heparin sodium and sodium citrate

CV

≤15.0%

LoD

The kit was validated by manufacturer’s LOD references with agreement rate 100%.

Specificity

Elevated interfering substances in specimen do not influence performance of the kit for detecting SARS-CoV-2 spike RBD antibody. The tested interfering substances included hemoglobin (500mg/dL), bilirubin (20mg/dL), triglyceride (1500 mg/dL), heterophil antibody (150U/mL), rheumatoid factors (100U/mL), 10%(v/v) human blood, phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (preservative included) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), Triamcinolone (2mg/mL), budesonide (2mg/mL) , Mometasone (2mg/mL), fluticasone (2mg/mL), Histamine Dihydrochloride (5mg/mL), ainterferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), Peramivir (1mg/mL) lopinavir (500mg/mL), ritonavir (1mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), cefprozil (40μg/mL) and meropenem (200mg/mL). Levofloxacin(10μg/mL), tobramycin (0.6mg/mL), EDTA (3mg/mL), Heparin Sodium (25U/mL), and Sodium Citrate (12mg/mL)

Applicable Instruments:

Universal microplate reader at wavelength 450nm/630nm.

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd. 


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