Enzyme-Linked Immunosorbent Assay for detecting SARS-CoV-2 Spike RBD Antibody

Short Description:

Enzyme-Linked Immunosorbent Assay for detecting SARS-CoV-2 Spike RBD Antibody was intended to detect the valence of Antibody of SARS-CoV-2 Spike RBD Antigen in serum/plasma from population inoculated by SARS-CoV-2 vaccine.

Product Detail

Product Tags

Product name

HWTS-RT055A-Enzyme-Linked Immunosorbent Assay for detecting SARS-CoV-2 Spike RBD Antibody




Coronavirus Disease 2019 (COVID-19) is a pneumonia caused by infection with a new coronavirus named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-CoV-2 was a strain in beta-CoV virus capsulated particulates in round or elliptical shape with diameters about 60nm-140nm. COVID-19 is an acute respiratory infectious disease, and the population is generally susceptible. Currently known infection source of COVID-19 are infected COVID-19 cases and asymptomatic carrier of SARS-CoV-2. Population inoculated by SARS-CoV-2 vaccine can generate spike RBD antibody or S antibody of SARS-CoV-2 detectable in serum and plasma, which can be as indicator to evaluate the efficiency of inoculating SARS-CoV-2 vaccine.

Technical Parameters




12 months

Specimen Type

human serum, plasma, samples with anticoagulant of EDTA, heparin sodium and sodium citrate




The kit was validated by manufacturer’s LOD references with agreement rate 100%.


Elevated interfering substances in specimen do not influence performance of the kit for detecting SARS-CoV-2 spike RBD antibody. The tested interfering substances included hemoglobin (500mg/dL), bilirubin (20mg/dL), triglyceride (1500 mg/dL), heterophil antibody (150U/mL), rheumatoid factors (100U/mL), 10%(v/v) human blood, phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (preservative included) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), Triamcinolone (2mg/mL), budesonide (2mg/mL) , Mometasone (2mg/mL), fluticasone (2mg/mL), Histamine Dihydrochloride (5mg/mL), ainterferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), Peramivir (1mg/mL) lopinavir (500mg/mL), ritonavir (1mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), cefprozil (40μg/mL) and meropenem (200mg/mL). Levofloxacin(10μg/mL), tobramycin (0.6mg/mL), EDTA (3mg/mL), Heparin Sodium (25U/mL), and Sodium Citrate (12mg/mL)

Applicable Instruments:

Universal microplate reader at wavelength 450nm/630nm.

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd. 

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