Hepatitis B Virus Genotyping Detection Kit (Fluorescent PCR)

Short Description:

This kit is used for qualitative typing detection of type B, type C and type D in the positive serum/plasma samples of hepatitis B virus (HBV)


Product Detail

Product Tags

Product name

HWTS-HP001-Hepatitis B Virus Genotyping Detection Kit (Fluorescent PCR)

Epidemiology

At present, ten genotypes from A to J of HBV have been identified worldwide. Different HBV genotypes have differences in epidemiological characteristics, virus variation, disease manifestations and treatment response, etc., which will affect the HBeAg seroconversion rate, the severity of liver lesions, and the incidence of liver cancer to a certain extent, and affect the clinical prognosis of HBV infection and the therapeutic efficacy of antiviral drugs to a certain extent.

Channel

Channel
Name
Reaction Buffer 1 Reaction Buffer 2
FAM HBV-C HBV-D
VIC/HEX HBV-B Internal Control

PCR Amplification Conditions

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 2 mins No
95℃ 5 mins No
2 40 cycles 95℃ 15 secs No
60℃ 45 secs Yes

Technical Parameters

Storage ≤-18℃ In dark 
Shelf-life 12 months
Specimen Type Serum, Plasma
Ct ≤38
CV ≤5.0%
LoD 1×10 2 IU/mL
Applicable Instruments It can match the mainstream fluorescent PCR instruments on the market.ABI 7500 Real-Time PCR Systems

ABI 7500 Fast Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

LightCycler®480 Real-Time PCR Systems

LineGene 9600 Plus Real-Time PCR Detection Systems

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System


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