Herpes simplex virus type 1/2,(HSV1/2) nucleic acid detection kit (Fluorescence PCR)

Short Description:

This kit is used for in vitro qualitative detection of Herpes Simplex Virus Type 1 (HSV1) and Herpes Simplex Virus Type 2 (HSV2) to help diagnose and treat patients with suspected HSV infections.


Product Detail

Product Tags

Product name

HWTS-UR018A-Herpes simplex virus type 1/2, (HSV1/2) nucleic acid detection kit (Fluorescence PCR)

Epidemiology

Sexually transmitted diseases (STD) are still one of the major threats to global public health security. Such diseases can lead to infertility, premature fetal delivery, tumor and various serious complications. There are many types of STD pathogens, including bacteria, viruses, chlamydia, mycoplasma and spirochetes, among which Neisseria gonorrhoeae, Mycoplasma genitalium, Chlamydia trachomatis, HSV1, HSV2, Mycoplasma hominis, and Ureaplasma urealyticum are common.

Genital herpes is a common sexually transmitted disease caused by HSV2, which is highly infectious. In recent years, the incidence of genital herpes has increased significantly, and due to an increase in risky sexual behaviors, the detection rate for HSV1 in genital herpes has increased and was reported to be as high as 20%-30%. An initial infection with the genital herpes virus is mostly silent without obvious clinical symptoms except local herpes in the mucosa or skin of a few patients. Since genital herpes is characterized by lifelong viral shedding and proneness towards recurrence, it is important to screen the pathogens as soon as possible and block its transmission.

Channel

FAM HSV1
CY5 HSV2
VIC(HEX) Internal Control

Technical Parameters

Storage Liquid: ≤-18℃ In dark
Shelf-life 12 months
Specimen Type urethral secretions, cervical secretions
Ct ≤38
CV ≤5.0%
LoD 50 Copies/reaction
Specificity There is no cross-reactivity with other STD pathogens such as Treponema pallidum, Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum. 
Applicable Instruments It can match the mainstream fluorescent PCR instruments on the market.

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd. 


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