Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Fluorescence PCR)

Short Description:

This kit is used for qualitative detection of herpes simplex virus type 2 nucleic acid in male urethral swab and female cervical swab samples.


Product Detail

Product Tags

Product Name

HWTS-UR007A-Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Fluorescence PCR)

Intended Use

This kit is used for qualitative detection of herpes simplex virus type 2 nucleic acid in male urethral swab and female cervical swab samples.

Epidemiology

Herpes Simplex Virus Type 2 (HSV2) is a circular virus synthesized with tegument, capsid, core, and envelope, and contains double-stranded linear DNA. Herpes virus can enter the body through direct contact or sexual contact with skin and mucous membranes, and is divided into primary and recurrent. Reproductive tract infection is mainly caused by HSV2, male patients are manifested as penile ulcers, and female patients are manifested as cervical, vulvar, and vaginal ulcers. The initial infections of genital herpes virus are mostly recessive infections, except for a few local herpes with mucous membranes or skin, most of which have no obvious clinical symptoms. Genital herpes infection has the characteristics of lifelong virus carrying and easy recurrence, and both patients and carriers are the source of infection of the disease. In China, the serological positive rate of HSV2 is about 10.80% to 23.56%. The stage of HSV2 infection can be divided into primary infection and recurrent infection, and about 60% of HSV2 infected patients relapse. 

Epidemiology

FAM: Herpes Simplex Virus Type 2 (HSV2)·

VIC(HEX): Internal Control

 

PCR Amplification Conditions Setting

Step

Cycles

Temperature

Time

Collect Fluorescent Signals or Not

1

1 Cycle

50℃

5mins

No

2

1 Cycle

95℃

10mins

No

3

40 Cycles

95℃

15secs

No

4

58℃

31secs

Yes

Technical Parameters

Storage  
Liquid

 ≤-18℃ In dark  

Shelf-life 12 months
Specimen Type

 Female cervical swab, Male urethral swab

Ct

≤38

CV

≤5.0%

LoD 50Copies/reaction
Specificity

There is no cross-reactivity with other STD pathogens, such as Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium and etc.

Applicable Instruments

It can match the mainstream fluorescent PCR instruments on the market.

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System.

Work Flow

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