Human cytomegalovirus (HCMV) nucleic acid detection kit (Fluorescence PCR)

Short Description:

This kit is used for qualitative determination of nucleic acids in samples including serum or plasma from patients with suspected HCMV infection, so as to help the diagnosis of HCMV infection.


Product Detail

Product Tags

Product name

HWTS-UR008A-Human cytomegalovirus (HCMV) nucleic acid detection kit (Fluorescence PCR)

Epidemiology

Human cytomegalovirus (HCMV) is a member with the largest genome in the herpes virus family and can encode more than 200 proteins. HCMV is narrowly restricted in its host range to humans, and there is still no animal model of its infection. HCMV has a slow and long replication cycle to form an intranuclear inclusion body, and trigger the production of perinuclear and cytoplasmic inclusion bodies and cell swelling (giant cells), hence the name. According to the heterogeneity of its genome and phenotype, HCMV can be divided into a variety of strains, among which there are certain antigenic variations, which, however, are of no clinical significance.

HCMV infection is a systemic infection, which clinically involves multiple organs, has complex and diverse symptoms, is mostly silent, and can cause a few patients to develop multiple-organ lesions including retinitis, hepatitis, pneumonia, encephalitis, colitis, monocytosis, and thrombocytopenic purpura. HCMV infection is very common and appears to spread worldwide. It is highly prevalent in the population, with incidence rates of 45-50% and more than 90% in developed and developing countries, respectively. HCMV can lay dormant in the body for a long time. Once the body's immunity is weakened, the virus will be activated to cause diseases, especially recurrent infections in leukemia patients and transplant patients, and can cause transplanted organ necrosis and endanger the life of patients in severe cases. In addition to stillbirth, miscarriage and premature delivery via intrauterine infection, cytomegalovirus can also cause congenital malformations, so HCMV infection is able to affect prenatal and postnatal care and population quality.

Channel

FAM HCMV DNA
VIC(HEX) Internal Control

Technical Parameters

Storage

Liquid: ≤-18℃ In dark  

Shelf-life

12 months

Specimen Type

Serum Sample, Plasma Sample

Ct

≤38

CV

≤5.0%

LoD

50 Copies/reaction

Specificity

There is no cross-reactivity with hepatitis B virus, hepatitis C virus, human papilloma virus, herpes simplex virus type 1, herpes simplex virus type 2, normal human serum samples, etc.

Applicable Instruments:

It can match the mainstream fluorescent PCR instruments on the market.

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd. 


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