Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene

Short Description:

The kit is intended for in vitro qualitative detection of the methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 genes in intestinal exfoliated cells in human stool samples.


Product Detail

Product Tags

Product name

HWTS-OT077-Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene Detection Kit (Fluorescence PCR)

Certificate

CE

Epidemiology

In adults, more than 10 8 intestinal epithelial cells fall off the intestinal wall every day, and are excreted with the feces through large bowel peristalsis. Due to the tumor cells are more likely to fall off the intestinal tract of the abnormal proliferation, the stool of intestinal tumor patients contains many diseased cells and abnormal cell components, which is the material basis for stable stool detection. Studies have found that methylation modification of gene promoters is an early event in tumorigenesis, and genetic material obtained from stool samples of colorectal cancer patients can reflect the presence of cancer in the intestine earlier.

NDRG4, also known as SMAP-8 and BDM1, is one of the four members of the NDRG gene family (NDRG1-4), which has been shown to be associated with cell proliferation, differentiation, development and stress. It is verified that NDRG4 methylation is a potential biomarker for non-invasive detection of colorectal cancer in stool samples.

SEPT9 is a member of the Septin gene family, which consists of at least 13 genes that encode a conserved GTPase domain that can bind cytoskeleton-related proteins, and is associated with cell division and tumorigenesis. Studies have found that the methylated Septin9 gene content is characteristically increased in stool samples from patients with colorectal cancer.

Secreted frizzled-related proteins (sFRPs) are soluble proteins that are a class of Wnt pathway antagonists due to their high structural homology to the frizzled (Fz) receptor for Wnt signaling. Inactivation of the SFRP gene results in uncontrolled activation of Wnt signaling associated with colorectal cancer. Currently, SFRP2 methylation in stool can be used as a non-invasive biomarker for the diagnosis of colorectal cancer.

BMP3 is a member of the TGF-B superfamily and thus plays an important role in embryonic development by inducing and shaping early bone formation. BMP3 is hypermethylated in colorectal cancer and can be used as an important tumor marker.

SDC2 is a cell surface heparan sulfate proteoglycan involved in the regulation of many physiological and pathological processes. Physical processed include cell proliferation, differentiation, adhesion, cytoskeletal organization, migration, wound healing, cell-matrix communication, angiogenesis; Pathological processes include inflammation and cancer. The methylation level of SDC2 gene in colorectal cancer tissues was significantly higher than that in normal tissues.

Channel

Reaction buffer A

VIC/HEX methylated NDRG4 gene
ROX methylated SEPT9 gene
CY5 internal control

Reaction buffer B

VIC/HEX methylated SFRP2 gene
ROX methylated BMP3 gene
FAM methylated SDC2 gene
CY5 internal control

Interpretation

Gene

Signal Channel

Ct Value

Interpretation

NDRG4

VIC (HEX)

Ct value≤38

NDRG4 positive

Ct value>38 or unde

NDRG4 negative

SEPT9

ROX

Ct value≤38

SEPT9 positive

Ct value>38 or unde

SEPT9 negative

SFRP2

VIC (HEX)

Ct value≤38

SFRP2 positive

Ct value>38 or unde

SFRP2 negative

Technical Parameters

Storage Liquid: ≤-18℃
Shelf-life 9 months
Specimen Type Stool Sample
CV ≤5.0%
Specificity There is no cross-reactivity with liver cancer, bile duct cancer, thyroid cancer and lung cancer
Applicable Instruments QuantStudio ®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler 

Work Flow

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006). 


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