Human Papillomavirus (28 Types) Genotyping

The kit uses multiple nucleic acid amplification (PCR) fluorescence detection method. Highly specific primers and probes are designed based on the L1 gene target sequence of HPV. The specific probe is labeled with FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore (HPV35, 44, 45, 54, 56, 68, 82) and ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) at 5’, and the 3’ quencher group is BHQ1 or BHQ2. During the PCR amplification, specific primers and probes bind to their respective target sequences. When Taq enzyme encounters the probes bound to the target sequence, it exerts the function of 5’ end exonuclease to separate the reporter fluorophore from the quencher fluorophore, so that the fluorescence monitoring system can receive the fluorescent signal, that is, every time a DNA strand is amplified, a fluorescent molecule is formed, which realizes the complete synchronization of the accumulation of fluorescent signals and the formation of PCR products, so as to achieve qualitative and genotyping detection of nucleic acids of 28 types of human papillomavirus in cervical exfoliated cell samples.