Human Papillomavirus (28 Types) Genotyping Detection Kit (Fluorescence PCR)

Short Description:

This kit is used for qualitative and genotyping detection of nucleic acid of 28 types of human papillomavirus (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) in female cervical exfoliated cells.


Product Detail

Product Tags

Product Name

HWTS-CC004A-Human Papillomavirus (28 Types) Genotyping Detection Kit (Fluorescence PCR)

Product Name

HWTS-CC004A-Human Papillomavirus (28 Types) Genotyping Detection Kit (Fluorescence PCR)

Epidemiology

The kit uses multiple nucleic acid amplification (PCR) fluorescence detection method. Highly specific primers and probes are designed based on the L1 gene target sequence of HPV. The specific probe is labeled with FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore (HPV35, 44, 45, 54, 56, 68, 82) and ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) at 5’, and the 3’ quencher group is BHQ1 or BHQ2. During the PCR amplification, specific primers and probes bind to their respective target sequences. When Taq enzyme encounters the probes bound to the target sequence, it exerts the function of 5’ end exonuclease to separate the reporter fluorophore from the quencher fluorophore, so that the fluorescence monitoring system can receive the fluorescent signal, that is, every time a DNA strand is amplified, a fluorescent molecule is formed, which realizes the complete synchronization of the accumulation of fluorescent signals and the formation of PCR products, so as to achieve qualitative and genotyping detection of nucleic acids of 28 types of human papillomavirus in cervical exfoliated cell samples.

Channel

FAM

16,58,53,73,6,26,40·

VIC/HEX

18,33,51,59,11,81,43

ROX 31,66,52,39,83,61,42
CY5 56,35,45,68,54,44,82

Technical Parameters

Storage ≤-18℃ In dark
Shelf-life

12 months

Specimen Type

 Cervical exfoliated cells
Ct ≤25
CV

≤5.0%

LoD

25 copies/reaction

Applicable Instruments  Easy Amp Real-time Fluorescence Isothermal Detection System(HWTS1600) Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).

Option 2.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).


  • Previous:
  • Next:

  • Write your message here and send it to us