Human Papillomavirus (28 Types) Genotyping

Short Description:

This kit is used for qualitative and genotyping detection of nucleic acid of 28 types of human papillomavirus (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) in male/female urine and female cervical exfoliated cells, providing auxiliary means for the diagnosis and treatment of HPV infection.


Product Detail

Product Tags

Product name

HWTS-CC013-Human Papillomavirus (28 Types) Genotyping Detection Kit (Fluorescence PCR)

Epidemiology

Cervical cancer is one of the most common malignant tumors of female reproductive tract. Previous studies have shown that persistent infection and multiple infection of human papillomavirus is one of the important causes of cervical cancer. At present, there is still a lack of recognized effective treatment means for HPV, so early detection and early prevention of cervical HPV are the keys to block cancer. To establish a simple, specific and rapid etiological diagnosis method is of great significance in the clinical diagnosis of cervical cancer.

Channel

Reaction buffer FAM VIC/HEX ROX CY5
HPV Genotyping Reaction Buffer 1 16 18 / Internal control
HPV Genotyping Reaction Buffer 2 56 / 31 Internal control
HPV Genotyping Reaction Buffer 3 58 33 66 35
HPV Genotyping Reaction Buffer 4 53 51 52 45
HPV Genotyping Reaction Buffer 5 73 59 39 68
HPV Genotyping Reaction Buffer 6 6 11 83 54
HPV Genotyping Reaction Buffer 7 26 44 61 81
HPV Genotyping Reaction Buffer 8 40 43 42 82

Technical Parameters

Storage Liquid: ≤-18℃
Shelf-life 12 months
Specimen Type Cervical Swab、Vaginal Swab、Urine
Ct ≤28
CV ≤5.0%
LoD 300Copies/mL
specificity All results are negative when the kit is used to detect non-specific specimens that have cross reactions with it, including ureaplasma urealyticum, chlamydia trachomatis of reproductive tract, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella and other HPV types not covered by the kit.
Applicable Instruments Applied Biosystems 7500 Real-Time PCR System

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR Systems

LineGene 9600 Plus Real-Time PCR Detection Systems

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Option 1.

Recommended extraction reagents: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8)

Option 2.

Recommended extraction reagents: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)


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