Human Respiratory Syncytial Virus Nucleic Acid

Short Description:

This kit is used for in vitro qualitative detection of Human respiratory syncytial virus (HRSV) nucleic acid in throat swab samples.

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Product Detail

Product Tags

Product name

HWTS-RT121-Human Respiratory Syncytial Virus Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)

HWTS-RT122-Freeze-dried Human Respiratory Syncytial Virus Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)

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Epidemiology

Human respiratory syncytial virus (HRSV), HRSV belongs to the Pneumoviridae and Orthopneumirus genus, a non-segmental single-stranded negative-stranded RNA virus. HRSV mainly causes respiratory tract infection and has become one of the main causes of hospitalization for respiratory tract infections in infants and children under 5 years old, and one of the main pathogens of severe respiratory diseases in adults, the elderly and immunocompromised patients.

Channel

FAM	HRSV nucleic acid
ROX	Internal Control

Technical Parameters

Storage	Liquid: ≤-18℃ In dark, Lyophilized: ≤30℃ In dark
Shelf-life	Liquid: 9 months, Lyophilized: 12 months
Specimen Type	Throat swab
Tt	≤40
CV	≤10.0%
LoD	1000Copies/mL
Specificity	No cross-reactivity with Human Coronavirus SARSr-CoV/ MERSr-CoV/ HCoV-OC43/ HCoV-229E/ HCoV-HKU1/ HCoV-NL63/ H1N1/ New influenza A (H1N1) virus (2009)/ Seasonal H1N1 influenza virus/ H3N2/ H5N1/ H7N9, Influenza B Yamagata/ Victoria, Parainfluenza 1/ 2/ 3, Rhinovirus A/ B/ C, Adenovirus 1/ 2/ 3/ 4/ 5/ 7/ 55, Human Metapneumovirus, Enterovirus A/ B/ C/ D, Human metapneumovirus, Epstein-Barr virus, measles virus, human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella-zoster virus, mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, Bacillus pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata, Pneumocystis jirovecii and Cryptococcus neoformans nucleic acids.
Applicable Instruments	Applied Biosystems 7500 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system Easy Amp Real-time Fluorescence Isothermal Detection System（HWTS1600）

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Kit(YD315-R) manufactured by Tiangen Biotech(Beijing) Co., Ltd.

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