STD Multiplex Detection Kit (Fluorescence PCR)

Short Description:

This kit is intended for qualitative detection of common pathogens of urogenital infections, including Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU), Herpes Simplex Virus Type 1 (HSV1), Herpes Simplex Virus Type 2 (HSV2), Mycoplasma hominis (Mh), Mycoplasma genitalium ( Mg) in male urinary tract and female genital tract secretion samples.


Product Detail

Product Tags

Product Name

HWTS-UR012A-STD Multiplex Detection Kit(Fluorescence PCR)

Epidemiology

Sexually transmitted diseases (STD) remain one of the major threats to global public health security, which can lead to infertility, preterm birth, tumorigenesis, and various serious complications. There are many types of STD pathogens, including bacteria, viruses, chlamydia, mycoplasma, and spirochetes. NG, CT, UU, HSV 1, HSV 2, Mh, Mg are more common.

Channel

Reaction Buffer

Target

Reporter

STD Reaction Buffer 1 

CT

FAM

UU

VIC(HEX)

Mh

ROX

HSV1

CY5

STD Reaction Buffer 2 

NG

FAM

HSV2

VIC(HEX)

Mg

ROX

IC

CY5

Technical Parameters

Storage Liquid: ≤-18℃ In dark
Shelf-life 12 months
Specimen Type urethral secretions, cervical secretions
Ct ≤38
CV ≤5.0%
LoD 50Copies/reaction
Specificity There is no cross-reactivity with other STD-infected pathogens such as Treponema pallidum.
Applicable Instruments

It can match the mainstream fluorescent PCR instruments on the market.

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR systems

SLAN® -96P Real-Time PCR Systems

LightCycler® 480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd. 


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