Influenza B Virus Nucleic Acid Detection Kit (Isothermal Amplification)

Short Description:

This kit in intended for in vitro qualitative detection of Influenza B virus nucleic acid in nasopharyngeal and oropharyngeal swab samples.


Product Detail

Product Tags

Product name

HWTS-RT127A-Influenza B Virus Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)

HWTS-RT128A-Freeze-dried Influenza B Virus Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)

Certificate

CE

Epidemiology

Influenza virus, a representative species of Orthomyxoviridae, is a pathogen that seriously threatens human health and can widely infect hosts. Seasonal influenza epidemics infect about 600 million people worldwide and cause 250,000 to 500,000 deaths every year , of which influenza B virus is one of the main causes[1]. Influenza B virus, also known as IVB, is a single-stranded negative-stranded RNA. According to the nucleotide sequence of its antigenic characteristic HA1 region, it can be divided into two major lineages, the representative strains are B/Yamagata/16/88 and B/Victoria /2/87(5)[2]. Influenza B virus generally has strong host specificity. It has been found that IVB can only infect humans and seals, and generally does not cause a worldwide pandemic, but it can cause regional seasonal epidemics [3]. Influenza B virus can be transmitted through various routes such as the digestive tract, respiratory tract, skin damage and conjunctiva. The symptoms are mainly high fever, cough, runny nose, myalgia, etc. Most of them are accompanied by severe pneumonia, severe heart attack. In severe cases, heart, kidney and other organ failure leads to death, and the fatality rate is very high [4]. Therefore, there is an urgent need for a simple, accurate and rapid method for detecting influenza B virus, which can provide guidance for clinical medication and diagnosis.

Channel

FAM IVB nucleic acid
ROX Internal Control

Technical Parameters

Storage

Liquid: ≤-18℃ In dark  

Lyophilization: ≤30℃ In dark

Shelf-life

Liquid: 9 months

Lyophilization: 12 months

Specimen Type

Nasopharyngeal swab samples

Oropharyngeal swab samples

CV

≤10.0%

Tt

≤40

LoD

1 Copy/µL

Specificity

there is no cross-reactivity with Influenza A, Staphylococcus aureus, Streptococcus (including Streptococcus pneumoniae), Adenovirus, Mycoplasma pneumoniae, Respiratory Syncytial Virus, Mycobacterium tuberculosis, Measles, Haemophilus influenzae, Rhinovirus, Coronavirus, Enteric Virus, swab of healthy person.

Applicable Instruments:

Applied Biosystems 7500 Real-Time PCR Systems

SLAN ® -96P Real-Time PCR Systems

LightCycler® 480 Real-Time PCR system

Easy Amp Real-time Fluorescence Isothermal Detection System(HWTS1600)

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd. 


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