Middle East Respiratory Syndrome Coronavirus Nucleic Acid

Short Description:

The kit is used for qualitative detection of MERS coronavirus nucleic acid in the nasopharyngeal swabs with Middle East Respiratory Syndrome (MERS) coronavirus.


Product Detail

Product Tags

Product name

HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)

Epidemiology

Middle East respiratory syndrome coronavirus (MERS-CoV), a β-coronavirus that causes respiratory disease in humans, was first identified in a deceased 60-year-old Saudi Arabian male patient on 24th July, 2012. The clinical presentation of MERS-CoV infection ranges from asymptomatic state or mild respiratory symptoms to severe acute respiratory disease even death.

Channel

FAM MERS virus RNA
VIC(HEX)

Internal Control

Technical Parameters

Storage

≤-18℃ In dark  

Shelf-life

9 months

Specimen Type

Freshly collected nasopharyngeal swabs

CV

≤5.0%

Ct

≤38

LoD

1000 Copies/mL

Specificity

There is no cross-reactivity with human coronaviruses human coronavirus SARSr-CoV and other common pathogens.

Applicable Instruments:

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Option 1.

Recommended extraction reagent: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) by Tiangen Biotech (Beijing) Co., Ltd. 

Option 2.

Recommended extraction reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). 


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