Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit (Fluorescent PCR)

Short Description:

The kit is used for qualitative detection of MERS coronavirus nucleic acid in the nasopharyngeal swabs and serum samples of patients infected with Middle East Respiratory Syndrome (MERS) coronavirus.


Product Detail

Product Tags

Product name

HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)

Epidemiology

Middle East respiratory syndrome coronavirus (MERS-CoV), a β-coronavirus that causes respiratory disease in humans, was first identified in a deceased 60-year-old Saudi Arabian male patient on 24th July, 2012. The clinical presentation of MERS-CoV infection ranges from asymptomatic state or mild respiratory symptoms to severe acute respiratory disease even death.

Channel

FAM MERS virus RNA
VIC(HEX)

Internal Control

Technical Parameters

Storage

≤-18℃ In dark  

Shelf-life

9 months

Specimen Type

Freshly collected nasopharyngeal swabs and serum samples

CV

≤5.0%

Ct

≤38

LoD

103Copies/mL

Specificity

There is no cross-reactivity with human coronaviruses human coronavirus SARSr-CoV and other common pathogens.

Applicable Instruments:

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd. 


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