Mycobacterium Tuberculosis INH Resistance

Short Description:

This kit is used to qualitatively detect the gene mutation of the 315th amino acid of the katG gene (K315G>C) and the gene mutation of the promoter region of the InhA gene (- 15 C>T).

Product Detail

Product Tags

Product Name

HWTS-RT002A-Mycobacterium Tuberculosis Isoniazid Resistance Detection Kit (Fluorescence PCR)


Isoniazid, a key anti-tuberculosis drug introduced in 1952, is one of the most effective drugs for combined treatment of active tuberculosis and a single drug for latent tuberculosis.

KatG is the main gene encoding catalase-peroxidase and katG gene mutation can promote the synthesis of mycolic acid cell wall, making the bacteria resistant to isoniazid. KatG expression is negatively correlated with changes in INH-MIC, and a 2-fold decrease in katG expression results in a slightly larger 2-fold increase in MIC. Another cause of isoniazid resistance in mycobacterium tuberculosis occurs when base insertion, deletion or mutation occurs in the InhA gene locus of mycobacterium tuberculosis.


ROX  inhA (-15C>T) site·

 katG (315G>C) site



Technical Parameters

Storage  ≤-18℃ In dark

12 months

Specimen Type


CV ≤5.0%

 1 × 103 bacteria/mL

Specificity  No-cross reactivity with the mutations of the four drug resistance sites (511, 516, 526 and 531) of the rpoB gene outside the detection range of the detection kit.

Applicable Instruments:

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Work Flow


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