Mycobacterium Tuberculosis Rifampicin Resistance Detection Kit (Fluorescence PCR)

Short Description:

This kit is suitable for the qualitative detection of the homozygous mutation in the 507-533 amino acid codon region of the rpoB gene that causes Mycobacterium tuberculosis rifampicin resistance. 

Product Detail

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Product name

HWTS-RT074A-Mycobacterium Tuberculosis Rifampicin Resistance Detection Kit (Fluorescence PCR) 


Rifampicin has been widely used in the treatment of pulmonary tuberculosis patients since the late 1970s, and has a significant effect. It has been the first choice to shorten the chemotherapy of pulmonary tuberculosis patients. Rifampicin resistance is mainly caused by the mutation of the rpoB gene. Although new anti-tuberculosis drugs are constantly coming out, and the clinical efficacy of pulmonary tuberculosis patients has also continued to improve, there is still a relative lack of anti-tuberculosis drugs, and the phenomenon of irrational drug use in clinical is relatively high. Obviously, the Mycobacterium tuberculosis in patients with pulmonary tuberculosis cannot be completely killed in a timely manner, which eventually leads to different degrees of drug resistance in the patient's body, prolongs the course of the disease, and increases the risk of death of the patient. This kit is suitable for the auxiliary diagnosis of Mycobacterium tuberculosis infection and the detection of rifampicin resistance gene, which is helpful to understand the drug resistance of mycobacterium tuberculosis infected by patients, and to provide auxiliary means for clinical medication guidance. 

Technical Parameters


≤-18℃ In dark  


9 months

Specimen Type





rifampicin-resistant wild type: 2x10 3 bacteria/mL

homozygous mutant: 2x10 3 bacteria/mL


This kit has no cross-reaction with human genome, other non-tuberculous mycobacteria, and pneumonia pathogens. It detects the mutation sites of other drug resistance genes of wild-type mycobacterium tuberculosis such as katG 315G>C\A, InhA-15C> T, the test results show no resistance to rifampicin, which means there is no cross-reaction.

Applicable Instruments:

SLAN-96P Real-Time PCR Systems

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd. 

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