Mycobacterium Tuberculosis Nucleic Acid and Rifampicin Resistance Detection Kit (Melting Curve)

Short Description:

This kit is suitable for the qualitative detection of Mycobacterium tuberculosis DNA in human sputum samples in vitro, as well as the homozygous mutation in the 507-533 amino acid codon region of the rpoB gene that causes Mycobacterium tuberculosis rifampicin resistance. 

Product Detail

Product Tags

Product name

HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid and Rifampicin Resistance Detection Kit (Melting Curve)




Mycobacterium tuberculosis , shortly as Tubercle bacillus, TB, is the pathogenic bacterium that causes tuberculosis. Currently, the commonly used first-line anti-tuberculosis drugs include isoniazid, rifampicin and hexambutol, etc. The second-line anti-tuberculosis drugs include fluoroquinolones, amikacin and kanamycin, etc. The new developed drugs are linezolid, bedaquiline and delamani, etc. However, due to the incorrect use of anti-tuberculosis drugs and the characteristics of the cell wall structure of mycobacterium tuberculosis, mycobacterium tuberculosis develops drug resistance to anti-tuberculosis drugs, which brings serious challenges to the prevention and treatment of tuberculosis.

Rifampicin has been widely used in the treatment of pulmonary tuberculosis patients since the late 1970s, and has a significant effect. It has been the first choice to shorten the chemotherapy of pulmonary tuberculosis patients. Rifampicin resistance is mainly caused by the mutation of the rpoB gene. Although new anti-tuberculosis drugs are constantly coming out, and the clinical efficacy of pulmonary tuberculosis patients has also continued to improve, there is still a relative lack of anti-tuberculosis drugs, and the phenomenon of irrational drug use in clinical is relatively high. Obviously, the Mycobacterium tuberculosis in patients with pulmonary tuberculosis cannot be completely killed in a timely manner, which eventually leads to different degrees of drug resistance in the patient's body, prolongs the course of the disease, and increases the risk of death of the patient. 



Channels and Fluorophores

Reaction Buffer A

Reaction Buffer B

Reaction Buffer C

FAM Channel

Reporter: FAM, Quencher: None

rpoB 507-514

rpoB 513-520

38KD and IS6110

CY5 Channel

Reporter: CY5, Quencher: None

rpoB 520-527

rpoB 527-533


HEX (VIC) Channel

Reporter: HEX (VIC), Quencher: None

Internal control

Internal control

Internal control

Technical Parameters


≤-18℃ In dark  


9 months

Specimen Type





mycobacterium tuberculosis 50 bacteria/mL

rifampicin-resistant wild type: 2x10 3 bacteria/mL

homozygous mutant: 2x10 3 bacteria/mL


It detects wild-type mycobacterium tuberculosis and the mutation sites of other drug resistance genes such as katG 315G>C\A, InhA-15C> T, the test results show no resistance to rifampicin, which means there is no cross-reactivity.

Applicable Instruments:

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

Work Flow


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