This kit is suitable for qualitative detection of the main mutation sites in human sputum samples collected from Tubercle bacillus positive patients that lead to mycobacterium tuberculosis isoniazid resistance: InhA promoter region -15C>T, -8T>A, -8T>C; AhpC promoter region -12C>T, -6G>A; homozygous mutation of KatG 315 codon 315G>A, 315G>C .