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SARS-CoV-2 Variants Detection Kit (Fluorescence PCR)

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Category:COVID-19 Test

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SARS-CoV-2 Variants Detection Kit (Fluorescence PCR)

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Introduction

[Product Name]

SARS-CoV-2 Variants Detection Kit (Fluorescence PCR

 

[Packaging Size]

50 tests/kit

 

[Intended Use]

This kit is intended to in vitro qualitative detection of novel coronavirus (SARS- CoV-2) in nasopharyngeal and oropharyngeal swab samples. RNA from SARS-CoV-2 is generally detectable in respiratory specimens during the acute phase of infection or asymptomatic people. It can be used further qualitative detection and differentiation of Alpha, Beta, Gamma, Delta and Omicron. 

The novel coronavirus (SARS-CoV-2) was discovered in Wuhan, China in December 2019, and has spread on a large scale around the world. In the process of dissemination, new mutations constantly occur, resulting in new variants. This product is mainly used for the auxiliary detection and differentiation of cases related to infection after the large-scale spread of Alpha, Beta, Gamma, Delta and Omicron mutant strains since December 2020. To carry out the nucleic acid detection of the new coronavirus, biosafety work should be done well.

 

[Main Components]

Components50 tests/kit

Specification

Quantity

Details

SARS-2-M Reaction Buffer 1

1.2mL /Vial

1Vial

Primers and probes for detecting N501Y, P681H, E484K, 211-212del sites and other reagents

SARS-2-M Reaction Buffer 2

1.2mL /Vial

1Vial

Primers and probes for detecting K417N, L452R, HV69-70del sites and other reagents

SARS-2-M Enzyme Mix

150μL /Vial

1Vial

 

Taq DNA polymerase, reverse transcriptaseUracil-DNA Glycosylase

SARS-2-M Positive Control

750μL/Vial

1Vial

Target gene pseudovirus and internal Conrol pseudovirus mixture

SARS-2-M Blank Control

750μL/Vial

1Vial

DNase/RNase free H2O

 

Note: Components of different batches cannot be mixed or interchanged.

 

[Storage Condition and Shelf-life]

The kit should stored below -18℃ protected from light. The shelf-life is 9 months.

The number of repeated freezing and thawing should not be more than 4 cycles.

After the kit is opened, please use it up within 3 months.

The kit can be stored stably for 7 days when transported protected from light below -18°C.

 

[Applicable Instruments]

QuantStudio™ 5 Real-Time PCR Systems, SLAN ® -96P Real-Time PCR Systems(Shanghai Hongshi Medical Technology Co., Ltd. ), LightCycler® 480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection SystemFQD-96ABioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) , BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System.

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