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Zika Virus Nucleic Acid Detection Kit(Fluorescence PCR)

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Category:Infectious Diseases

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Zika Virus Nucleic Acid Detection Kit(Fluorescence PCR)

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Introduction

[Product Name]

Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)

 

[Packaging Size]

50 tests/kit

 

[Intended Use]

This kit is used to qualitatively detect Zika virus nucleic acid in serum samples of patients suspected of Zika virus infection in vitro, and provide an auxiliary means for the diagnosis of patients with Zika virus disease. The test results are for clinical reference only, and the final diagnosis should be comprehensively considered in combination with other clinical indicators. The use should comply with the "Zika Virus Disease Prevention and Control Program (First Edition)" issued by the National Health and Family Planning Commission and relevant laboratory testing requirements.

Zika virus belongs to the genus Flaviviridae, is a single-stranded positive-stranded RNA virus[1] with a diameter of 40-70nm. It has an envelope, contains 10794 nucleotides, and encodes 3419 amino acids[2]. According to the genotype, it is divided into African type and Asian type. Zika virus disease is a self-limiting acute infectious disease caused by the Zika virus, which is mainly transmitted through the bite of Aedes aegypti mosquitoes[3]. The clinical features are mainly fever, rash, arthralgia or conjunctivitis, and it is rarely fatal[4]. According to the World Health Organization, neonatal microcephaly and Guillain-Barre syndrome (Guillain-Barré syndrome) may be associated with Zika virus infection.

 

[Main Components]

 

S/N

Component(50 tests/kit)

Strength

Quantity

Component Description

1

Zika Reaction Buffer

1.2mL/vial

1 vial

Amplification reaction reagents, primer probes, internal reference primer probes, etc.

2

Zika Enzyme Mix

75μL/vial

1 vial

Taq enzyme, reverse transcriptase, UDG enzyme, etc.

3

Zika Positive Control

600μL/vial

1 vial

Mixed solution of viral target gene and internal reference

4

Zika Blank Control

600μL/vial

1 vial

DNase/RNase free H2O

 

Note: Components of different batches cannot be mixed or interchanged.

 

[Storage Conditions and Shelf-life]

This kit should be stored under -18°C and protected from light, and the shelf life is 9 months (please use within the shelf life). After opening, the number of repeated freezing and thawing times is no more than 4 cycles, and it can be stored stably for 5 days when transported below -18°C and protected from light.

See the packaging label for the production date, production batch number and expiration date.

 

[Applicable Instruments]

Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems(Shanghai Hongshi Medical Technology Co., Ltd. ), LightCycler®480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection System(FQD-96A,Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) , BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System.

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