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Freeze-dried Vibrio cholerae O1 and Enterotoxin Gene Nucleic Acid Detection Kit(Fluorescence PCR)

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Category:Gastrointestinal diseases

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Freeze-dried Vibrio cholerae O1 and Enterotoxin Gene Nucleic Acid Detection Kit(Fluorescence PCR)

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Introduction

[Product Name]

Freeze-dried Vibrio Cholerae O1 and Enterotoxin Gene Nucleic Acid Detection Kit(Fluorescence PCR)

 

[Packing Size]

20 tests/kit, 50 tests/kit

 

[Intended Use]

This kit is suitable for qualitative detection of vibrio cholerae type O1 and enterotoxin gene nucleic acid in stool samples of patients with acute gastroenteritis, and provides an effective auxiliary means for clinical diagnosis and treatment of cholera.

Vibrio cholerae is a severe intestinal infectious disease caused by cholera toxin. It has the characteristics of rapid onset, rapid spread and wide epidemic range, and is one of the Class A infectious diseases under international quarantine. According to the difference of O antigen of vibrio cholerae, it can be divided into 139 serogroups. There are two main serotypes that can cause cholera, O1 group and O139 group. Seven cholera pandemics worldwide were caused by vibrio cholerae O1. The strong pathogenic effect of cholera is mainly caused by the cholera toxin (CT) secreted by vibrio cholerae. The cholera toxin is encoded by the gene ctx, so in cholera O1 group, the bacteria that do not produce cholera toxin CT will not cause cholera. The establishment of a simple, specific and rapid pathogenic diagnostic method is of great significance for the clinical diagnosis of vibrio cholerae.

 

[Main Components]

S/N

Component(20 tests/kit)

Character

Quantity(Strength)

Component Description

1

Cholera Reaction Buffer

 freeze-dried          

1 bottle

Contains VCO1, VCCTX and internal reference specific primers, fluorescent probes, reaction buffer, Taq enzyme and UDG enzyme, etc.

2

 

Cholera Positive Control

freeze-dried

1 vial

VCO1, VCCTX and internal reference plasmid were diluted to appropriate concentrations and mixed in equal proportions

3

Cholera Blank Control

liquid

1 vial, 600μL/vial

DNase/RNase free H2O

4

Reconstituted Solution

liquid

1 vial, 1mL/vial

DNase/RNase free H2O,etc.

 

S/N

Component(50 tests/kit)

Character

Quantity(Strength)

Component Description

1

Cholera Reaction Buffer

 freeze-dried         

1 bottle

Contains VCO1, VCCTX and internal reference specific primers, fluorescent probes, reaction buffer, Taq enzyme and UDG enzyme, etc.

2

Cholera Positive Control

freeze-dried

1 vial

VCO1, VCCTX and internal reference plasmid were diluted to appropriate concentrations and mixed in equal proportions

3

Cholera Blank Control

liquid

1 vial, 600μL/vial

DNase/RNase free H2O

4

Reconstituted Solution

liquid

 

1 vial, 1.45mL/vial

DNase/RNase free H2O,etc.

Note: Components of different batches cannot be interchanged.

 

[Storage Conditions and Shelf-life]

1.Unopened kit: It should be stored at no higher than 30℃ and protected from light, and the shelf-life is 12 months.

2.RT-PCR detection should be performed immediately after reconstitution of the lyophilized reaction buffer, otherwise it should be stored at -25℃ to -15℃ and protected from light. The validity period is

3.After reconstituting the lyophilized positive control, it should be stored at -25°C to -15°C. The validity period is 1 week, and repeated freezing and thawing should be avoided (limited to 2 cycles).

4.Transportation conditions: It can be transported stably for 1 month when the temperature is lower than 37℃.

5.See the packaging label for the production date, production batch number and expiration date.

 

[Applicable Instruments]

QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems(Shanghai Hongshi Medical Technology Co., Ltd. ), LightCycler®480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection System(FQD-96A,Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) , BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System.

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