中文版
js_thumb bannerPic
/
/
/
/
HWTS-CC008A 14 High-Risk HPV with 16/18 Genotyping Test Kit(Fluorescence PCR)

Product category

All categories

Contact info

Category:Reproductive health testing

1/1
Number of views:
1000

HWTS-CC008A 14 High-Risk HPV with 16/18 Genotyping Test Kit(Fluorescence PCR)

Retail price
0.0
Market price
0.0
Number of views:
1000
Quantity
-
+
Stock:
0
1
Introduction

[REF] HWTS-CC008A
[Product Name]14 High-Risk HPV with 16/18 Genotyping Test Kit(Fluorescence PCR)
[Packaging Size] 50 tests/kit
[Research Use Only]
The kit is used for qualitative fluorescence-based PCR detection of nucleic acid fragments specific to 14 human papillomavirus (HPV) types (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) in cervical exfoliated cells in women, as well as for HPV 16/18 genotyping to detect HPV infection.
Cervical cancer is one of the most common malignant tumors in the female reproductive tract. It has been shown that persistent HPV infection and multiple infections are one of major causes of cervical cancer. 
[Test principle]
The kit uses multiplex fluorescence-based qPCR. It contains highly specific primers and probes designed according to target sequences in the HPV L1 gene. The specific probes are labeled with four distinct fluorophores, FAM (HPV 18), VIC (16), ROX (HPV31,33,35,39,45,51,52,56,58,59,66 and 68) and CY5 (Internal Control) at the 5'-end, and a quencher (BHQ1 or BHQ2) at the 3’-end. During PCR amplification, the specific primers and probes separately bind to their own target sequences. When the Taq DNA polymerase encounters the probes binding to their target sequences, it exhibits the exonuclease activity at the 5'-end to isolate the fluorophores (reporter dyes) from the quencher, thereby allowing the fluorescence-monitoring system to receive fluorescence signals, i.e., when a DNA strand is amplified, a fluorescent molecule will be formed to achieve complete synchronization of PCR product formation and fluorescence signal accumulation, thereby qualitatively detecting nucleic acid fragments specific to 14 HPV types (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 ,68) in cervical exfoliated cells.
[Storage Conditions and Shelf-life]
The kit should be stored below -18°C and protected from light, the shelf life is 12 months. After opening the container, please use it up within 3 months. It shall not be subjected to more than 4 freezing-thawing cycles.
[Applicable Instruments]
SLAN ® -96P Real-Time PCR Systems, Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio™ 5 Real-Time PCR Systems, LightCycler® 480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A), MA-6000 Real-Time Quantitative Thermal Cycler.
[Specimen Requirements]
1.    Specimen collection
Before sampling, use a cotton swab to gently wipe the excessive secretions from the cervix. Discard the used swab. Allow a new cotton swab infiltrated with cell preservation solution or a brush for sampling cervical exfoliated cells to adhere closely to the cervical mucosa, and rotate it clockwise for 3-5 times to obtain cervical exfoliated cells. Slowly take out the cotton swab or brush and put it in 1 mL sterile saline. After fully rinsing it, squeeze the cotton swab or brush against the tube wall to dry it and then discard it. Tighten the tube cap and mark the name (or number) of the specimen on the tube.
2.    Storage
Specimens to be tested should not be stored for more than 48h at 2-8°C or for more than six months below -18°C; they can be stored for a long time at -70°C. Avoid repeated thawing and freezing.
3.    Transportation
It is recommended to use liquid nitrogen and dry ice for ultra-low temperature transportation.
 

We could not find any corresponding parameters, please add them to the properties table

Relevant attachments

Previous page
1

RELATED PRODUCTS

产品展示
Search
Search

Suzhou Office: Building 1, No. 188, Fuchunjiang Road, High-tech Zone, Suzhou
Beijing Office: Building 2, No. 28, Yuhua Road, Airport Science and Technology Park, Shunyi District, Beijing
Contact number:
+86-10-80497001
Nantong Office: No. 888 Zhujiang Road, Juegang Street, Rudong County, Jiangsu Province
Contact number:
+86-513-80562882(Administration & Human Resources)

                            +86-512-6515-8191(Marketing & Sales)
Company email: sales@hongweitest.com

Macro micro-test

WeChat

Copyright © 2021 Jiangsu Macro & Micro-Test Med-Tech Co.,Ltd.苏ICP备18057655号 苏公网安备 32062302000215号 Power by:300.cn
Suspended
返回顶部