中文版
js_thumb bannerPic
/
/
/
/
HWTS-CC009A Human Papillomavirus (28 Types) Nucleic Acid Test Kit (Fluorescence PCR)

Product category

All categories

Contact info

Category:Reproductive health testing

1/1
Number of views:
1000

HWTS-CC009A Human Papillomavirus (28 Types) Nucleic Acid Test Kit (Fluorescence PCR)

Retail price
0.0
Market price
0.0
Number of views:
1000
Quantity
-
+
Stock:
0
1
Introduction

[REF] HWTS-CC009A

[Specification] 50 tests/kit

[Research Use Only]

This kit is used for qualitative detection of 28 types of human papillomavirus (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) in female cervical exfoliated cells, but is not used for completely genotyping. 

Cervical cancer is one of the most common malignant tumors of female reproductive tract. Previous studies have shown that persistent infection and multiple infection of human papillomavirus is one of the important causes of cervical cancer. To establish a simple, specific and rapid etiological detection method is of great significance in cervical cancer.

[Test Principles]

The kit uses multiple nucleic acid amplification (PCR) fluorescence detection method. Highly specific primers and probes are designed based on the L1 gene target sequence of HPV. The specific probe is labeled with FAM fluorophore (HPV6, 11, 16, 18, 31, 54, 56, 83), VIC fluorophore (HPV26, 44, 61, 81 and internal control), CY5 fluorophore (HPV40, 42, 43, 45, 51, 52, 53, 82) or ROX fluorophore (HPV33, 39, 35, 58, 59, 66, 68, 73) at 5', and the 3' quencher group is BHQ1 or BHQ2. During the PCR amplification, specific primers and probes bind to their respective target sequences. When Taq enzyme encounters the probes bound to the target sequence, it exerts the function of 5' end exonuclease to separate the reporter fluorophore from the quencher fluorophore, so that the fluorescence monitoring system can receive the fluorescent signal, that is, every time a DNA strand is amplified, a fluorescent molecule is formed, which realizes the complete synchronization of the accumulation of fluorescent signals and the formation of PCR products, so as to achieve qualitative detection of 28 types of human papillomavirus (HPV 6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) in cervical exfoliated cell samples.

[Storage Conditions and Shelf Life]

This kit should be stored below-18°C and protected from light. Its shelf life is 9 months. After opening, please use it up within 3 months. It shall not be subjected to repeated freezing and thawing for more than 4 cycles. It shalt be transported below-18°C and protected from light, and can be stably stored for 5 days.

[Applicable Equipment]

Applied Biosystems 7500 Real-Time PCR Systems, Ultrassay XP96 Real Time qPCR System

[Acceptable Specimens]

Before sampling, gently wipe the excessive secretions of the cervix with a cotton swab, replace the cotton swab, use a cotton swab infiltrated with cell preservation solution or a sampling brush for cervical exfoliated cells to close to the cervical mucosa, and turn clockwise for 3-5 cycles to obtain cervical exfoliated cells. Slowly take out the cotton swab or brush, and put into the sample tube with 1 ml of sterile saline. After rinsing thoroughly, squeeze the cotton swab or brush against the wall and discard. Tighten the cap, and mark the sample name (or number) and type on the sample tube.

2. Storage

The sample n be tested should not be stored at 2-8°C for more than 48 h, should be stored below-18℃ for no More than six months, and can be stored below-70°C for a long time. Avoid repeated freezing and thawing.

3. Transportation

It is recommended to adopt ultra-low temperature transportation with liquid nitrogen and dry ice.

We could not find any corresponding parameters, please add them to the properties table

Relevant attachments

Previous page
1

RELATED PRODUCTS

产品展示
Search
Search

Suzhou Office: Building 1, No. 188, Fuchunjiang Road, High-tech Zone, Suzhou
Beijing Office: Building 2, No. 28, Yuhua Road, Airport Science and Technology Park, Shunyi District, Beijing
Contact number:
+86-10-80497001
Nantong Office: No. 888 Zhujiang Road, Juegang Street, Rudong County, Jiangsu Province
Contact number:
+86-513-80562882(Administration & Human Resources)

                            +86-512-6515-8191(Marketing & Sales)
Company email: sales@hongweitest.com

Macro micro-test

WeChat

Copyright © 2021 Jiangsu Macro & Micro-Test Med-Tech Co.,Ltd.苏ICP备18057655号 苏公网安备 32062302000215号 Power by:300.cn
Suspended
返回顶部