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HWTS-UR021A Herpes Simplex Virus 1&2 Nucleic Acid Detection Kit (Fluorescence PCR)

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HWTS-UR021A Herpes Simplex Virus 1&2 Nucleic Acid Detection Kit (Fluorescence PCR)

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Introduction

[REF] HWTS-UR021A

[Specification] 50 tests/kit

[Research Use Only]

This kit is used for qualitative detection of Herpes Simplex Virus Type 1 (HSV1) and Herpes Simplex Virus Type 2 (HSV2) to help detect patients with suspected HSV infections [1-2].

Sexually transmitted diseases (STD) are still one of the major threats to global public health security. Such diseases can lead to infertility, premature fetal delivery, tumor and various serious complications [3-6]. There are many types of STD pathogens, including bacteria, viruses, chlamydia, mycoplasma and spirochetes, among which Neisseria gonorrhoeae, Mycoplasma genitalium, Chlamydia trachomatis, HSV1, HSV2, Mycoplasma hominis, and Ureaplasma urealyticum are common.

Genital herpes is a common sexually transmitted disease caused by HSV2, which is highly infectious. In recent years, the incidence of genital herpes has increased significantly, and due to an increase in risky sexual behaviors, the detection rate for HSV1 in genital herpes has increased and was reported to be as high as 20%-30%. An initial infection with the genital herpes virus is mostly silent without obvious klinical symptoms except local herpes in the mucosa or skin of a few patients. Since genital herpes is characterized by lifelong viral shedding and proneness towards recurrence, it is important to screen the pathogens as soon as possible and block its transmission.

[Test Principles]

This kit uses PCR combined with Taqman fluorescent probes. It contains specific primers and probes for fluorescence detection, which are designed to target the conserved regions of HSV1 and HSV2. The HSV1-specific probes are labeled with the fluorophore FAM at the 5'-end, and the quencher BHQ1 at the 3’-end, respectively. The HSV2-specific probes are labeled with the fluorophore CY5 at the 5'-end, and the quencher BHQ3 at the 3’-end, respectively. During PCR amplification, the specific primers and probes separately bind to their own target sequences. When the Taq DNA polymerase encounters the probes binding to their target sequences, it exhibits the exonuclease activity at the 5'-end to isolate the fluorophores (reporter dyes) from the quencher, thereby allowing the fluorescence-monitoring system to receive fluorescence signals, i.e., when a DNA strand is amplified, a fluorescent molecule will be formed to achieve complete synchronization of PCR product formation and fluorescence signal accumulation, thereby qualitatively detecting HSV1 and HSV2 in a sample.

The kit contains internal control to control the quality of reagents, DNA and the operation itself to avoid false negative test results.

[Storage Conditions and Shelf Life]

This kit should be stored below -18, and its shelf life is 12 months. After opening and using the kit, store the remaining reagents below -18 protected from light. It shall not be subjected to repeated freezing and thawing for more than 4 cycles. After opening it, use it up within 3 months. It can be stored stably for 5 days when it is transported below -18°C under dark conditions.

[Applicable Equipment]

Applied Biosystems 7500 Real-Time PCR Systems, BioRad CFX96 Real- Time PCR System, Ultrassay Archimed X4/X6 Real Time qPCR System

[Acceptable Specimens]

1. Sample collection

Male: Take a urethral secretion, or insert a small cotton swab about 2-4cm into the urethra, then slightly twist it to take out a secretion (which should slightly contain a mucosa). Put the secretion or cotton swab into a sterile tube, then seal it tightly with a sterile cotton ball or rubber stopper, and send it for testing.

Female: Cervix — Use a cotton ball moistened with sterile normal saline to wipe off secretions outside the cervix, then insert a sterile cotton swab into the cervix, and let it stop there for 5 seconds, then slightly twist it to take a cervical secretion (which should slightly contain a mucosa). Put the cotton swab into a sterile tube, then seal it tightly with a sterile cotton ball or rubber stopper, and send it for testing. Urethra — Use a cotton ball moistened with sterile normal saline to clean the urethral opening, then insert a sterile cotton swab about 2cm into the urethra, and slightly twist it to take out a secretion (which should slightly contain a mucosa). Put the cotton swab into a sterile tube, then seal it tightly with a sterile cotton ball or rubber stopper, and send it for testing.

2. Sample storage

Samples to be tested should be stored at 2-8°C for no more than 7 days, at -18°C for no more than 7 months, or at -70°C for a longer time.

Samples should not be subjected to repeated thawing and freezing.

3. Sample transportation

Samples are transported for no more than 5 days using airtight foam boxes with dry ice.

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