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HWTS-HP008A HBV DNA Quantitative Detection Kit (Fluorescence PCR)

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Category:Hepatitis

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HWTS-HP008A HBV DNA Quantitative Detection Kit (Fluorescence PCR)

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Introduction

[REF] HWTS-HP008A

[Specification] 50 tests/kit

[Research Use Only]

The HBV PCR Kit is an nucleic acid amplification test for the quantitation of hepatitis B virus (HBV) in human serum.

Hepatitis B is an infectious disease with liver and multiple organ lesion caused by the hepatitis B virus (HBV).  Most people experience symptoms such as extreme fatigue, appetite loss, lower limbs or whole-body edema, hepatomegaly, etc. 5% of adult patients and 95% of children patients infected from their mother cannot clean the HBV virus efficiently in persistent infection and progress to liver cirrhosis or primary liver cell carcinoma[1-5].

[Test Principle]

A pair of primers and a sequence-specific fluorescence probe were designed. The fluorophore FAM is attached at the 5' end of the probe and the quencher moiety TAMRA is located at the 3' end. During the PCR procedures, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye when the probes hybridize to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye. The detectable fluorescence is proportional to the amount of accumulated PCR product, which is used to detect the HBV quantitatively.

[Storage Conditions and Shelf Life]

The components of the HBV PCR Kit should be stored at temperature lower than -18°C and are stable with self-life about 12 months. Repeated thawing and freezing should be avoided more than 5 times.

The dates of manufacturing and expiry stated on the label.

[Applicable Equipment]

Applied Biosystems™ Real time PCR system 7500, ABI 7500FAST real-time PCR system, ABI QuantStudio™5 Real-time PCR system, LightCycler® 480 fluorescence quantitative PCR system, Bio-Rad CFX96 real-time fluorescence quantitative PCR instrument, Ultrassay™ XP96 Real Time qPCR System. etc.

[Acceptable Specimens]

Collection:

A volume of 2mL venous blood should be withdrawn using sterile syringe needle and standard specimen collection tubes (Sterile centrifuge tube). Freshly specimens (whole blood) may be held at ambient temperature (2 to 30°C) for up to 4 hours prior to centrifugation. After the blood is centrifuged at 1,600rpm for 20 minutes, the isolated serum should be transferred into another sterile centrifuge tube for future test.

Storage:

Serum should be stored at 2-8°C for up to 24 hours, at -18°C no more than 3 months, and at -70°C for long term. The frozen specimens should be thawed completely before the test. Multiple freeze-thaw cycles should be avoided (<5 x).

Transportation

Ship the specimens in sealed frozen or foam box on dry ice.

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