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HWTS-OT067A HIV RNA Detection Kit (Fluorescence PCR)

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HWTS-OT067A HIV RNA Detection Kit (Fluorescence PCR)

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Introduction

[REF]HWTS-OT067A

[Specification] 50 tests/kit

[Research Use Only]

HIV RNA Detection Kit (Fluorescence PCR) (hereinafter referred to as the Kit) is used for the qualitative detection of human immunodeficiency virus RNA in human serum or plasma samples.

The human immunodeficiency virus (HIV), which lives in a person's blood, can destroy the body's immune system , thus making the body lose its ability to fight off other diseases , causing incurable infections and tumors, and eventually death. HIV can be transmitted through sexual contact, blood and from mother to child.

This kit is suitable for the qualitative detection of human immunodeficiency virus RNA in serum/ plasma samples.

This kit should not be used for blood source screening.

 

[Test Principles]

This kit uses a combination of PCR amplification and fluorescence probe to design specific detection primers and fluorescence probe for the conserved regions of human immunodeficiency  virus . The probe was an oligonucleotide  labeled with 5' FAM fluorescence  group and 3' BHQ quenching group . When the probe was intact, the fluorescence signal emitted by the reporter group was absorbed by the quenched group . During PCR amplification , the 5'-3' exonuconase activity of Taq enzyme will degrade the probe enzyme and separate the reporting  and quenching  fluorophore,  so that the fluorescence signal  can be received  by the fluorescence  monitoring  system . That is, for each amplified DNA strand, a fluorescence molecule is formed, and the accumulation of fluorescence signal is fully synchronized  with the formation  of PCR products . The extracted RNA was reverse transcribed into cDNA, and fluorescence PCR detection technology was used to detect the nucleic acid in the sample by the change of fluorescence signal in the PCR process and the change of fluorescence  signal value of RNA standard  amplification. At the same time, exogenous internal control was added to control the quality of reagent, DNA and operation to avoid false negative.

 

[Storage Conditions and Shelf Life]

This kit should be stored in dark conditions below -18℃ for 9 months (please use within the validity period). After opening, repeated melting times shall not be more than 4 times.

It can be stably stored for up to 5 days under the conditions of -18℃ under dark conditions. Production date and expiration date: see the packing box.

[Applicable Equipment]

Applied Biosystems 7500 Real-Time PCR Systems, BioRad CFX96 Real- Time PCR System, Ultrassay XP96 Real Time qPCR System

[Acceptable Specimens]

1. Sample collection

This kit is suitable for serum or plasma samples. When collecting serum samples, venous blood of the subject is taken.  Draw 2mL with a sterile injection needle and collect it in a sterile centrifuge tube, stored at room temperature for no more than 4 hours, centrifuged at 1600rpm for 20 minutes, extracted serum (do not inhale red blood cells) and transferred to another sterile centrifuge tube for standby. When collecting plasma samples, draw 2mL with a sterile injection needle and collect it in a sterile centrifuge tube containing EDTA as an anticoagulant (heparin was not used for anticoagulation), and stored at room temperature for no more than 4 hours, centrifuged at 1600rpm for 20 minutes at room temperature, separated plasma (do not inhale red blood cells), and transferred to a sterile centrifuge tube for reserve.

2. Sample storage

Samples to be tested should not be stored at 2-8℃ for more than a week; Store at -18℃ for no more than 6 months. The extraction of frozen samples should be carried out after the samples are completely melted and mixed. Repeated freezing and thawing should be avoided, and repeated freezing and thawing should not be more than 5 times.

3. Sample transportation

Use foam box with ice seal for transportation.

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