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HWTS-UR022A Cytomegalovirus Nucleic Acid Detection Kit (Fluorescence PCR)

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HWTS-UR022A Cytomegalovirus Nucleic Acid Detection Kit (Fluorescence PCR)

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Introduction

[REF] HWTS-UR022A

[Specification] 50 tests/kit

[Research Use Only]

Human cytomegalovirus (CMV) is a member with the largest genome in the herpes virus family and can encode more than 200 proteins. CMV is narrowly restricted in its host range to humans, and there is still no animal model of its infection. HCMV has a slow and long replication cycle to form an intranuclear inclusion body, and trigger the production of perinuclear and cytoplasmic inclusion bodies and cell swelling (giant cells), hence the name. According to the heterogeneity of its genome and phenotype, CMV can be divided into a variety of strains, among which there are certain antigenic variations, which, however, are of no significance.

CMV infection is a systemic infection, which involves multiple organs, has complex and diverse symptoms, is mostly silent, and can cause a few patients to develop multiple-organ lesions including retinitis, hepatitis, pneumonia, encephalitis, colitis, monocytosis, and thrombocytopenic purpura. CMV infection is very common and appears to spread worldwide. It is highly prevalent in the population, with incidence rates of 45-50% and more than 90% in developed and developing countries, respectively [1]. CMV can lay dormant in the body for a long time. Once the body's immunity is

weakened, the virus will be activated to cause diseases, especially recurrent infections in leukemia patients and transplant patients, and can cause transplanted organ necrosis and endanger the life of patients in severe cases [2-3]. In addition to stillbirth, miscarriage and premature delivery via intrauterine infection, cytomegalovirus can also cause congenital malformations, so CMV infection is able to affect prenatal and postnatal care and population quality.

This kit is used for qualitative determination of nucleic acids in samples including blood from patients with suspected CMV infection. The test results are for research use only and should be closely combined with data for final conclusion.

 

[Test Principle]

This kit uses PCR combined with fluorescent probes. It contains specific primers and probes for fluorescence detection, which are designed to target the highly conserved region of CMV. The fluorescent probes are labeled with FAM at the 5'-end and the quencher BHQ1 at the 3’-end. At the same time, the kit contains internal references and corresponding specific primers and probes, of which the probes are labeled with the fluorophore VIC (HEX) at the 5'-end and the quencher BHQ1 at the 3’-end. During PCR amplification, CMV-specific primers and probes bind to target sequences, and complete synchronization of PCR product formation and fluorescence signal accumulation is achieved based on the polymerase activity and 5’-3’ exonuclease activity of the Taq DNA polymerase, thereby realizing the qualitative detection of target nucleic acids in a sample.

 

[Storage Conditions and Shelf Life]

This kit should be stored below -18°C protected from light, and its shelf life is 12 months. It shall not be subjected to repeated freezing and thawing for more than 4 cycles, and it can be stored stably for 5 days when it is transported below -18°C under dark conditions.

[Applicable Equipment]

ABI 7500, ABI Stepone, Ultrassay XP96 Real Time qPCR System.

[Acceptable Specimens]

1.  Sample collection

Freshly collected human serum, plasma or urine samples from patients with suspected CMV infection

2.  Storage

The sample to be tested is stored below -70°C after collection, and repeated thawing and freezing is prohibited.

3.  Transportation

Samples are transported using airtight foam boxes with dry ice.

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