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HWTS-HP010A HCV Genotyping Detection Kit (Fluorescence PCR)

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Category:Hepatitis

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HWTS-HP010A HCV Genotyping Detection Kit (Fluorescence PCR)

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Introduction

[REF] HWTS-HP010A

[Specification] 50 tests/kit

[Research Use Only]

The HCV Genotyping Real-Time PCR Kit is used for detect and genotype Hepatitis C Virus (HCV) subtypes 1b, 2a, 3a, 3b and 6a in human blood plasma or serum samples. The test results are for laboratory reference only, and the final conclusion should be comprehensively considered in close combination with other results.

Hepatitis C virus (HCV) belongs to the flaviviridae family, Its genome is a single-stranded positive-stranded RNA, which is easily mutated. The virus exists in the hepatocytes, serum leukocytes and plasma of infected persons. The HCV gene is susceptible to mutation and can be divided into at least 6 genotypes and multiple subtypes. HCV genotypes 1b and 2a are relatively common in our country, among which 1b is the main type, followed by 2 and 3. No reports of genotypes 4 and 5 have been reported, and 6 is relatively rare.

[Test Principle]

This detection kit adopts the PCR method combined with the amplification detection technology of fluorescent probes, and selects the NS5B region of hepatitis C virus as the target sequence for detection [2~4]. Select the conserved sequences of different gene subtypes to design the primer probes for detection, and use different fluorescent groups for labeling [5]. Using the fluorescence quantitative PCR detection technology, the change of the fluorescent signal during the PCR process is used to realize the detection of hepatitis C in the sample. Detection of different genotypes of the virus. The detection system contains an internal standard, and the processing of the sample to be tested and whether there is a PCR inhibition reaction in the detection are monitored by whether the nucleic acid amplification of the internal standard is normal, so as to avoid false negatives in the detection.

 

[Storage Conditions and Shelf Life]

HCV RNA quantitation kit should be stored in dark at temperature lower than -18°C stable with self-life about 9 months.

Repeated freezing-thawing cycles (5 x) should be avoided.

 

[Applicable Equipment]

Applied Biosystems™ Real time PCR system 7500, ABI 7500FAST real-time PCR system, ABI QuantStudio™5 Real-time PCR system, LightCycler® 480 fluorescence quantitative PCR system, Bio-Rad CFX96 real-time fluorescence quantitative PCR instrument, Ultrassay XP96 Real Time qPCR System. etc.

[Acceptable Specimens]

Specimen collection

Human blood plasma and serum are the most suitable sample materials for HCV detection. 2mL venous blood should be withdraw on the appropriate parts of the elbow bend, the forearm, or the back of the hand using sterile syringe and collected in standard specimen collection tubes or anticoagulation tubes (EDTA·K2 or Sodium citrate).

The whole blood should be centrifuged at 1600 rpm for 20 minutes within 4 hours to separate serum or plasma. The separated serum or plasma must be transferred to a sterile centrifuge tube. Be sure not to draw red cell in transferring.

Blood specimen in tube with Heparin sodium as anticoagulant cannot be used in the assay.

Storage The specimen should be kept at 2~8°C no longer than 1 week, -18°C for no longer than 6 months and -70°C for longer term preservation. Repeated freezing-thawing should be avoided.

Transportation

Specimen should be transported frozen using sealed container with ice bag or drikold.

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