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Freeze-dried Enterovirus Universal, EV71 and CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)

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Freeze-dried Enterovirus Universal, EV71 and CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)

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Introduction

[Product Name]
Freeze-dried Enterovirus Universal, EV71 and CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)

[Packaging Size] 20 tests/kit, 50 tests/kit

[Intended Use]
This kit is used for in vitro qualitative detection of enterovirus, EV71 and CoxA16 nucleic acids in oropharyngeal swabs and herpes fluid samples of patients with hand-foot-mouth disease, and provides an auxiliary means for the diagnosis of patients with hand-foot-mouth disease. Hand-foot-mouth disease is an infectious disease caused by enteroviruses. Currently, 108 serotypes of enteroviruses have been found, which are divided into four groups: A, B, C and D. Among them, enterovirus EV71 and CoxA16 are the main pathogens. The disease mostly occurs in children under 5 years old, and can cause herpes on the hands, feet, mouth and other parts, and a small number of children can cause complications such as myocarditis, pulmonary edema, aseptic meningoencephalitis, etc.
[Test Principles]

This kit uses a combination of PCR amplification and fluorescent probes. Design specific primers and probes for the highly conserved region of Enterovirus, EV71 and CoxA16. The probes of Enterovirus are labeled with FAM fluorescent groups, the probes of EV71 are labeled with ROX fluorescent groups, the probes of CoxA16 are labeled with VIC fluorescent groups. At the same time, an internal control is introduced, and a specific primer probe for fluorescence detection is designed. The 5' end of the probe is labeled as CY5 fluorophore, and the 3' end quenching group is BHQ2. In the process of PCR amplification, specific primer probes bind to their respective target sequences. When Taq enzyme encounters the probes bound to the target sequence, it exerts the function of 5′-end exonuclease, so that the reporter and the quencher is separated, and the fluorescence monitoring system can receive the fluorescent signal, that is, each time a DNA chain is amplified, a fluorescent molecule is formed, realizing the complete synchronization of the accumulation of the fluorescent signal and the formation of the PCR product. By detecting different fluorescence signal changes, realize the qualitative detection of Enterovirus, EV71 and CoxA16 nucleic acid in oropharyngeal swabs and herpes fluid samples of patients with hand-foot-mouth disease, and provide auxiliary means for the diagnosis and treatment of patients infected with Enterovirus, EV71 and CoxA16 virus.

[Storage Conditions and Shelf-life]

1.Unopened kit: It should be stored at no higher than 30°C and protected from light, and the shelf-life is 12 months.
2. RT-PCR detection should be performed immediately after reconstitution of the lyophilized reaction buffer and positive control, otherwise it should be stored at -25°C to -15°C and protected from light. The validity period is 1 week, and repeated freezing and thawing should be avoided ( limited to 2 cycles)
3.Transportation conditions: It can be transported stably for 1 month when the temperature is not higher than 37°C.

4.See the packaging label for the production date, production batch number and expiration date. [Applicable Instruments]
Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems(Shanghai Hongshi Medical Technology Co., Ltd. ), LightCycler®480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A,Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) , BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System.

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