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Human Sperm Concentration Detection Kit (Immunochromatography)

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Human Sperm Concentration Detection Kit (Immunochromatography)

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Introduction

[Product Name] Human Sperm Concentration Detection Kit (Immunochromatography)

[Packaging Size]

5 tests/kit, 20 tests/kit

[Intended Use]

This kit is suitable for qualitative detection of sperm concentration in human semen in vitro. The main factors affecting male infertility are: semen volume, total sperm count, sperm concentration, liquefaction time, semen pH value, sperm normal morphology rate, etc. Among them, sperm concentration detection is an important indicator to measure male infertility. The normal minimum value of sperm concentration is 15×106/ml. When the sperm concentration is less than 15×106/ml, it is considered to have reproductive difficulties. Based on several anatomical, genetic, and biochemical features, SP10 protein is shown to be a selectable analyte for sperm concentration, expressed only in the testis, a differentiation marker in the final step of the spermatogenesis process, and SP10 protein concentration and sperm concentration show a certain linear relationship. The concentration of SP10 protein in the sample can be detected as an indicator of sperm concentration.
[Test Principles]
This kit adopts the principle of double-antibody sandwich method of immunochromatography, uses anti-human sperm SP10 protein monoclonal antibody A for tracer labeling, and coats anti-human sperm SP10 protein monoclonal antibody B and quality control antibody on the nitrocellulose membrane detection line and the quality control line respectively. When testing, the semen sample should be processed first with the sample diluent, and the pretreated sample solution should be added dropwise to the sample area of the reagent sample pad, and move forward along the test strip under the action of chromatography. If there is a certain concentration of protein SP10 in the sample, the protein SP10 binds to the anti-human fFN monoclonal antibody A label to form a complex. Due to the chromatographic effect, the complex moves forward along the paper strip and binds to anti-human fFN monoclonal antibody B to form a labeling complex, which shows a red band at the detection line. When the sample does not contain protein SP10 or the protein SP10 concentration is lower than LoD, the detection line does not show a red band. Regardless of whether the sample contains the antigen to be tested or not, a red band should appear at the quality control line(C line), as an

internal control for whether the chromatography process is normal and whether the kit is invalid.

[Storage Conditions and Shelf-life]

Storage Conditions: 4-30°C.
Shelf-life: 12 months tentatively.
See label for production and expiry dates.

The test cassette should be used within 60 minutes after being taken out of the aluminum foil bag.

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