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Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)

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Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)

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Introduction

[Product Name] Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Enzymatic Probe

Isothermal Amplification)
[Packaging Size] 50 tests/kit
[Intended Use]
This kit is used for the qualitative detection of herpes simplex virus type 2 nucleic acid in genitourinary tract samples in vitro.

Herpes Simplex Virus Type 2 (HSV2) is a circular virus synthesized with envelope, capsid, core, and envelope, and contains double-stranded linear DNA [1]. Herpes virus can enter the body through direct contact with skin and mucous membranes or sexual contact, and is divided into primary and recurrent. Reproductive tract infection is mainly caused by HSV2, male patients manifest as penile ulcers, and female patients is cervical, vulvar, and vaginal ulcers. The initial infection of genital herpes virus is mostly a recessive infection. Except for a few herpes in mucous membranes or skin, most of them have no obvious clinical symptoms. Genital herpes infection has the characteristics of life-long and easy recurrence.Both patients and carriers are the source of infection of the disease [2]. In China, the seropositive rate of HSV2 is about 10.80% to 23.56%. The stage of HSV infection can be divided into primary infection and recurrent infection, and about 60% of HSV2 infected patients recur [3].

The test results of this kit are not the only indicator for the diagnosis of herpes simplex virus type 2 infection in patients. It must be combined with the clinical characteristics of the patient and other laboratory test data to correctly determine the infection or colonization of the pathogen, and formulate a reasonable treatment plan to make the treatment safe and effective.

[Test Principles]

This kit adopts Enzymatic Probe Isothermal Amplification (EPIA), and designs specific primers and RNA base-containing probes (rProbe) for the highly conserved region of HSV2, and adds Bst enzyme and RNaseH enzyme at the same time, in which the left and right ends of the RNA base of rProbe are labeled with fluorescent groups and quencher, respectively. Use the DNA polymerase activity and strand displacement activity of Bst enzyme to amplify the target to be tested at a constant temperature, the RNaseH enzyme can cleave the RNA bases on the target-probe hybrid chain, so that the fluorescent group and quencher of rProbe are separated thereby fluorescing. In addition, the left fragment of the residual rProbe RNA base can be used as a primer to further extend to form a product, which further accumulates the product. The fluorescent signal is

continuously accumulated with the formation of the product, thereby realizing the detection of the target nucleic acid.
In the system, the recombinant pine chloroplast gene plasmid is used as an exogenous internal reference to control the quality of the reagents and the operation itself to avoid false negatives.

[Storage Conditions and Shelf-life]

This kit is stored below -18°C and protected from light. The shelf life is 9 months. The repeated freezing and thawing times no more than 4 cycles. It can be stored stably for 5 days when it is transported in dark conditions below -18°C.
[Applicable Instruments]

Applied Biosystems 7500 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems(Shanghai Hongshi Medical Technology Co., Ltd. ), LightCycler®480 Real-Time PCR system,Easy Amp Real-time Fluorescence Isothermal Detection System(HWTS1600).

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