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Dengue NS1 Antigen, IgM/IgG Antibody Dual Detection Kit (Immunochromatography)

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Dengue NS1 Antigen, IgM/IgG Antibody Dual Detection Kit (Immunochromatography)

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Introduction

[Product Name] Dengue NS1 Antigen, IgM/IgG Antibody Dual Detection Kit (Immunochromatography)

[Packaging Size] 1 test/kit, 20 tests/kit

[Intended Use]

This kit is used for in vitro qualitative detection of dengue NS1 antigen and IgM/IgG antibody in serum, plasma and whole blood by immunochromatography, as an auxiliary diagnosis of dengue virus infection.
Dengue fever is an acute infectious disease caused by dengue virus, and it is also one of the most widely spread mosquito-borne infectious diseases in the world. Serologically, it is divided into four serotypes, DENV-1, DENV-2, DENV-3, and DENV-4[1]. The four serotypes of dengue virus often have alternate prevalence of different serotypes in a region, which increases the possibility of dengue hemorrhagic fever and dengue shock syndrome. With the increasingly serious global warming, the geographical distribution of dengue fever tends to spread, and the incidence and severity of the epidemic also increase. Dengue fever has become a serious global public health problem.

This product is a rapid, on-site and accurate detection kit for dengue virus NS1 antigen and IgM/IgG antibody. Specific IgM antibody indicates that there is a recent infection, but a negative IgM test does not prove that the body is not infected. It is also necessary to detect specific IgG antibodies with a longer half-life and the highest content to confirm the diagnosis. In addition, after the body is infected, the NS1 antigen appears first, so the simultaneous detection of the dengue virus NS1 antigen and specific IgM and IgG antibodies can effectively diagnose the body's immune response to a specific pathogen[2], and this antigen-antibody combined detection kit can perform rapid early diagnosis and screening in the early stage of dengue infection, primary infection and secondary or multiple dengue infection, shorten the window period and improve the detection rate.

[Test Principles]

This kit uses immunochromatography technology to detect dengue virus NS1 antigen by double antibody sandwich method and detect dengue virus IgM/IgG antibody by capture method.
The cassette includes dengue virus NS1 antigen detection strip and dengue virus IgM/IgG antibody detection strip. In dengue virus NS1 antigen detection strip, the T line on the NC membrane is coated with dengue fever NS1 antibody, the C line is coated with goat-anti-mouse IgG antibody as the quality control line, and the glass cellulose membrane is coated with the labeled dengue fever NS1 antibody complex. In dengue virus IgM/IgG antibody detection strip, the NC membrane is coated with anti-human IgM and anti-human IgG monoclonal antibodies, the C line is coated with goat- anti-mouse IgG antibody as the quality control line, and the glass cellulose membrane is coated with labeled dengue antigen complex.

During detection, drop the processed sample into the sample well of the test cassette. When the sample contains the target pathogen (dengue virus NS1 antigen, IgM/IgG antibody) and the concentration is higher than the LoD, the target pathogen reacts with the corresponding target-labeled antibody and antigen to form a complex. Under the action of chromatography, the complex moves forward along the nitrocellulose membrane, agglutinates at the detection line and finally forms a red band, indicating that the corresponding detection item is positive. On the contrary, if the sample does not contain the target pathogen or the antigen or antibody concentration is lower than the LoD, there will be no red band at the detection line, indicating that the corresponding detection item is negative. Regardless of whether the target pathogen exists in the sample, a red band should appear at the quality control line (C line) of the two test strips, as an internal control for whether the chromatography process is normal and whether the kit is invalid.

[Storage Conditions and Shelf-life]

Storage Conditions: 4-30°C. Try to use it within 1 hour after opening to avoid the

open time of the package too long to make the kit become invalid. Shelf-life: 12 months.
See label for production and expiry dates.

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