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Mycoplasma Hominis Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)

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Category:Reproductive health testing

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Mycoplasma Hominis Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)

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Introduction

[Product Name] Mycoplasma Hominis Nucleic Acid Detection Kit(Enzymatic Probe Isothermal

Amplification)
[Packaging Size] 50 tests/kit
[Intended Use]
This kit is used for the qualitative detection of mycoplasma hominis nucleic acid in genitourinary tract samples in vitro.
Mycoplasma hominis (Mh) is the smallest prokaryotic microorganism that can live independently between bacteria and viruses, and is also a pathogenic microorganism that is prone to genital and urinary tract infections. For men, it can cause prostatitis, urethritis, pyelonephritis, etc. For women, it can cause inflammatory reactions in the reproductive tract such as vaginitis, cervicitis, and pelvic inflammatory disease. It is one of the pathogens that cause infertility and abortion[1-2].
The test result of this kit is not the only indicator for the diagnosis of mycoplasma hominis infection in patients. The clinical characteristics of the patients and other laboratory test data must be combined to correctly determine the infection or colonization of the pathogen, and formulate a reasonable treatment plan to make the treatment safe and effective.
[Test Principles]
This kit adopts Enzymatic Probe Isothermal Amplification (EPIA), and designs specific primers and RNA base-containing probes (rProbe) for the highly conserved region of Mh, and adds Bst enzyme and RNaseH enzyme at the same time, in which the left and right ends of the RNA base of rProbe are labeled with fluorescent groups and quencher, respectively. Use the DNA polymerase activity and strand displacement activity of Bst enzyme to amplify the target to be tested at a constant temperature, the RNaseH enzyme can cleave the RNA bases on the target-probe hybrid chain, so that the fluorescent group and quencher of rProbe are separated thereby fluorescing. In addition, the left fragment of the residual rProbe RNA base can be used as a primer to further extend to form a product, which further accumulates the product. The fluorescent signal is continuously accumulated with the formation of the product, thereby realizing the detection of the target nucleic acid.
In the system, the recombinant pine chloroplast gene plasmid is used as an exogenous internal reference to control the quality of the reagents and the operation itself to avoid false negatives.

[Storage Conditions and Shelf-life]
This kit is stored below -18°C and protected from light. The shelf life is 9 months. The repeated freezing and thawing times no more than 4 cycles. It can be stored stably for 5 days when it is transported in dark conditions below -18°C.
[Applicable Instruments]
Applied Biosystems 7500 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems(Shanghai Hongshi Medical Technology Co., Ltd. ), LightCycler®480 Real-Time PCR system, Real-time Fluorescence Constant Temperature Detection System Easy Amp HWTS1600.

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