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Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

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Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

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Introduction

[Product Name] Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

[Packaging Size] 50 tests/kit
[Intended Use]
This kit is used for in vitro qualitative detection of monkeypox virus nucleic acid in human rash fluid, nasopharyngeal swabs, throat swabs and serum samples.
Monkeypox (MP) is an acute zoonotic infectious disease caused by Monkeypox Virus (MPV). MPV is round-bricked or oval in shape, and is a double-stranded DNA virus with a length of about 197Kb[1]. The disease is mainly transmitted by animals, and humans could become infected by being bitten by infected animals or by direct contact with the blood, body fluids and rash of infected animals. The virus can also be transmitted between people, primarily through respiratory droplets during prolonged, direct face-to-face contact or through direct contact with a patient’s body fluids or contaminated objects[2-3]. The clinical symptoms of monkeypox infection in humans are similar to those of smallpox, generally after a 12-day incubation period, appearing fever, headache, muscle and back pain, enlarged lymph nodes, fatigue and discomfort. The rash appears after 1-3 days of the fever, usually first on the face, but also in other parts. The disease course generally lasts 2-4 weeks, and the mortality rate is 1%-10%. Lymphadenopathy is one of the main differences between this disease and smallpox[4].
The test results of this kit are not used as the only indicator for the diagnosis of monkeypox virus infection in patients, which must be combined with the clinical characteristics of the patient and other laboratory test data to correctly determine the infection of the pathogen, and formulate a reasonable treatment plan to make the treatment safe and effective.

[Test Principles]

This kit adopts fluorescent probe real-time PCR technology, and select two specific conserved regions of monkeypox virus MPV-1 gene and MPV-2 gene to design specific primer probes. The MPV-1 gene probe 5’ is labeled with FAM, and the 3’ quenching group is labeled with BHQ1. The MPV-2 gene probe 5’ is labeled with VIC, and the 3’ quenching group is labeled with BHQ1. In the process of PCR amplification, specific primers and probes bind to their respective target sequences separately. When Taq enzyme encounters the probe bound to the target sequence, it exerts the function of 5’-end exonuclease, making the reporter fluorophore and the quenching group separated, thus the fluorescence monitoring system could receive the fluorescent signal, that is, a fluorescent

molecule is formed every time a DNA chain is amplified, realizing that the accumulation of the fluorescent signal is completely synchronized with the formation of the PCR product. At the same time, endogenous internal control quality control is introduced to avoid false negatives in the test.

[Storage Conditions and Shelf-life]

This kit should be stored below -18°C away from light, and the shelf-life is 12 months. The opening and repeated freezing and thawing should be no more than 4 cycles. The kit should be transported below -18°C away from light and can be stably stored for 5 days.
See the packaging label for the production date, production batch number and expiry date.

[Applicable Instruments]

QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Shanghai Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR systems, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR Systems, BioRad CFX Opus 96 Real-Time PCR Systems.

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