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Orthopox Virus Universal Type/Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

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Orthopox Virus Universal Type/Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

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Introduction

[Product Name] Orthopox Virus Universal Type/Monkeypox Virus Nucleic Acid Detection

Kit(Fluorescence PCR)
[Packaging Size] 50 tests/kit
[Intended Use]
This kit is used for in vitro qualitative detection of orthopox virus universal type and monkeypox virus nucleic acid in human rash fluid, nasopharyngeal swab, oropharyngeal swab and serum samples.

Poxviridae is a double-stranded DNA virus with the largest genome (175-225 kb) in the vira, of which orthopox virus (OPV) belongs to its first subgenus. Orthopox virus includes many viruses that can cause human disease, such as variola virus (VARV), monkeypox virus (MPV), cowpox virus (CPV) and vaccinia virus (VACV) ) and so on [1], in which both VARV and MPV can cause severe rash, fever and other clinical symptoms in the population, and are highly infectious [2]. The virus can also be transmitted from person to person, primarily through respiratory droplets during prolonged, direct face-to-face contact or through direct contact with a patient's body fluids or contaminated objects[3-4].

The test results of this kit are not the only indicator for the diagnosis of orthopox virus infection in patients. It must be combined with the clinical characteristics of the patient and other laboratory test data to correctly determine the infection of the pathogen, and formulate a reasonable treatment plan to make the treatment safe and effective.

[Test Principles]

This kit adopts fluorescent probe real-time PCR technology, and selects the specific conservative region of orthopox virus universal type to design specific primer probes. The 5' is labeled with FAM, and the 3' quencher is BHQ1. Selects the specific conservative region MPV-1 gene and MPV-2 gene of the monkeypox virus to design specific primer probes. MPV-1 gene probe 5' is labeled with ROX, 3' quencher is BHQ2. MPV-2 gene probe 5' is labeled with VIC, and the 3' quencher is BHQ1. In the process of PCR amplification, specific primers and probes bind to their respective target sequences. When Taq enzyme encounters the probe bound to the target sequence, it exerts the function of 5'-end exonuclease, making the reporter and the quencher separated, so that the fluorescence monitoring system can receive the fluorescent signal, that is, a fluorescent molecule is formed every time a DNA chain is amplified, realizing the complete synchronization of the accumulation of the fluorescent signal and the formation of the PCR product. At the same time, endogenous internal control is introduced to avoid false negatives in the test.

[Storage Conditions and Shelf-life]

The kit should be stored below -18°C protected from light. The shelf life is 12 months. The number of repeated freezing and thawing should not be more than 4 cycles. It can be stably stored for 5 days below -18°C and protected from light during transportation.
See the packaging label for the production date, batch number and expiration date.

[Applicable Instruments]

QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems(Shanghai Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection System(FQD-96A,Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) , BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System

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