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Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

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Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

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Introduction

[Product Name]

Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

[Packing Size]

20 tests/kit, 50 tests/kit

[Intended Use]

This kit is used for qualitative typing detection of denguevirus (DENV) nucleic acid in suspected patient’s serum sample to help diagnose patients with Dengue fever. The test results obtained by the kit are for clinical reference only. The final diagnosis results must be combined with the clinical manifestations of patients and other laboratory tests to conduct a comprehensive analysis of the disease condition.

Dengue fever (DF), which is induced by denguevirus (DENV) infection, is one of the most epidemic arbovirus infectious diseases. Its transmission medium includes Aedes aegypti and Aedes albopictus. DF is mainly prevalent in tropical and subtropical areas. DENV belongs to flavivirus under flaviviridae, and can be classified into 4 serotypes according to surface antigen. Clinical manifestations of DENV infection mainly include headache, fever, weakness, enlargement of lymph node and leukopenia, etc., and bleeding, shock, hepatic injury or even death in severe cases. In recent years, climate change, urbanization, quick development of tourism and other factors have provided more rapid and convenient conditions for transmitting and spreading of DF, leading to constant expansion of epidemic area of DF. Therefore, establishment of easy, specific and rapid etiological diagnosis method of DENV has important significance for clinical diagnosis of DF.

[Test Principle]

This kit uses PCR combined with Taqman fluorescent probes. It contains specific primers and probes for fluorescence detection, which are designed to target the specific conserved regions of DENV I/II/III/IV. The I/II/III/IV-specific probes are labeled with the reporter FAM/VIC(HEX)/ROX/CY5 at the 5’-end, and the quencher BHQ1 or BHQ2 at the 3’-end, respectively. The extracted RNA is reverse transcribed to cDNA.

In the process of PCR amplification, specific primers and probes bind to their respective target sequences. When Taq enzyme encounters the probe bound to the target sequence, it exerts the function of 5’-end exonuclease, making the reporter and quencher dyes separated, so that the fluorescence monitoring system can receive the fluorescent signal, that is, each time a DNA chain is amplified, a fluorescent molecule is formed, realizing the complete synchronization of the accumulation of the fluorescent signal and the formation of the PCR product. The qualitative detection of each gene type is realized by 1 tube of reaction buffer.

[Storage Conditions and Shelf Life]

Storage condition: This kit should be stored below -18°C away from light, and the shelf life is 12 months. The repeated freezing and thawing should be avoided (no more than 4 cycles).
It should be frozen below -18°C for storage after opening, and if stored at 2~8°C, please use it within a week.

Transport condition: The kit placed in a transport box containing dry ices can be stably stored for 5 days.

See the label for the production date, production batch number and expiry date.

[Applicable Instrument]

Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Shanghai Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection System(FQD-96A,Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) , BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System.

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