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18 Types of High-risk Human Papilloma Virus Nucleic Acid Detection Kit (Fluorescence PCR)

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18 Types of High-risk Human Papilloma Virus Nucleic Acid Detection Kit (Fluorescence PCR)

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Introduction

[Product Name]

18 Types of High-risk Human Papilloma Virus Nucleic Acid Detection Kit (Fluorescence PCR)
[Packing Size]
50 tests/kit

[Intended Use]

This kit is suitable for in vitro qualitative detection of 18 types of human papilloma viruses (HPV) (HPV16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) specific nucleic acid fragments in female cervical exfoliated cells and HPV 16/18 typing, to help diagnose and treat HPV infections.

Cervical cancer is one of the most common malignant tumors of the female reproductive tract. Studies have shown that HPV persistent infections and multiple infections are one of the main causes of cervical cancer. Currently, the recognized effective treatments are still lack for cervical cancer caused by HPV, so early discovery and prevention of cervical infection caused by HPV is the key to preventing cervical cancer. It is of great significance to establish a simple, specific and rapid etiology diagnostic test for the clinical diagnosis and treatment of cervical cancer.

The detection of kit cannot be applied alone or prior to cervical cytology, and it is not recommended to use this kit alone for cervical cancer screening in any age group. This test cannot replace cervical cytology, nor can it be used alone as the basis for patient management. Since the kit has not undergone corresponding clinical trials, it should not be used for clinical purposes related to cervical cancer screening.

[Test Principle]

This kit uses multiplex fluorescence quantitative PCR. It contains highly specific primers and probes designed based on the HPV L1 gene target sequence. Specific probes are labeled with different fluorophores at the 5’-end, FAM (HPV 18), VIC (HEX) (HPV16), ROX (HPV26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73 and 82) and CY5 (internal control), with a quenching fluorophore (BHQ1 or BHQ2) at the 3’-end. During PCR amplification, specific primers and probes bind to their respective target sequences separately. When Taq enzyme encounters the probe bound to the target sequence, it exerts 5’-end exonuclease activity, and the fluorescent group is separated from the quenching group, so that the fluorescence monitoring system can receive the fluorescent signal, that is, each time a DNA strand is amplified, a fluorescent molecule is formed, realizing the complete synchronization of PCR product formation and fluorescent signal accumulation, so as to qualitatively detect 18 types of human papilloma viruses (HPV16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) and provide auxiliary means for the diagnosis and treatment of patients with HPV infections.

[Storage Conditions and Shelf Life]

This kit should be stored below -18°C away from light, and the shelf life is 12 months. After opening, please use it up within 3 months. The repeated freezing and thawing should be no more than 4 cycles.

[Applicable Instrument]

SLAN-96P Real-Time PCR Systems (Shanghai Hongshi Medical Technology Co., Ltd.), Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, LightCycler®480 Real-Time PCR Systems, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Bioer technology) and MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.).

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