+86-512-6515-8191（Marketing & Sales） Nantong Office: No. 888 Zhujiang Road, Juegang Street, Rudong County, Jiangsu Province Suzhou Office: Building 1, No. 188, Fuchunjiang Road, High-tech Zone, Suzhou Beijing Office: Building 2, No. 28, Yuhua Road, Airport Science and Technology Park, Shunyi District, Beijing Contact Number:+86-10-80497001
[Product Name]Freeze-dried Human Parainfluenza Virus I Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)
[Packaging Size] 48 tests/kit
This kit is used for in vitro qualitative detection of parainfluenza virus type I nucleic acid in throat swab samples.
There are four types of human parainfluenza virus (HPIVs), which are common viruses that cause lower respiratory tract infection in children. The most typical clinical symptom caused by type I is laryngotracheobronchitis. It is the main cause of laryngotracheobronchitis, and type II is the second. Both types I and II can cause other upper and lower respiratory tract diseases. Type III mainly causes pneumonia and bronchiolitis. Type IV is difficult to detect and less likely to cause serious disease. The incubation period of human parainfluenza virus is generally about 1 to 7 days. The test results are not the only basis for the diagnosis of parainfluenza virus type I infection in patients. The clinical characteristics of the patient and other laboratory test data must be combined to correctly determine the infection or colonization of the pathogen, and formulate a reasonable treatment plan to make the treatment safe and effective.
The kit was to detect nucleic acid of HPIVIusing Enzymatic Probe Isothermal Amplification (EPIA). Primers and Probe with RNA bases (rProbe) were developed tailored to the highly conservative sequences in genomes of HPIV I and added Bst enzyme and RNaseH. Two ends of RNA bases in rProbe were labeled with fluorophores as reporter and quencher. During amplification, the target sequence was amplified in isothermal condition using DNA polymerase and strand replacement of Bst enzyme, RNaseH cleaves RNA bases on target gene-probe hybrid strands, enabling to separate the reporter dye from the quencher dye and to generate the fluorescent signal. The remaining segment of rProbe RNA can continue the amplification process to generate fluorescent signals further. The fluorescent signals can be monitored by detection system, thereby realizing the detection of the target nucleic acid.
Recombinant pseudo-virus of Pine chloroplast gene was developed as internal control in the kit to monitor the quality in laboratory processing including validity of reagent and laboratory operation to avoid false negative.
[Storage Condition and Shelf-life]
1. Unopened kit: It should be stored at no higher than 30°C and protected from light, and the shelf-life is 12 months.
2.Test should be performed immediately after reconstitution of the freeze-dried reaction buffer, internal control and positive control, the remaining internal control and positive control should be stored below -15°C and protected from light. The validity period is 1 week, and repeated freezing and thawing should be avoided ( limited to 2 cycles).
3.Transportation conditions: It can be transported stably for 1 month when the temperature is not higher than 37°C.
4. See the packaging label for the production date, production batch number and expiration date.
Applied Biosystems 7500 Real-Time PCR Systems, SLAN ® -96P Real-Time PCR Systems, LightCycler® 480 Real-Time PCR system, Easy Amp Real-time Fluorescence Isothermal Detection System(HWTS1600)
[Requirements for Samples]
1. Sample Collection
Freshly collected throat swabs.
Wipe the posterior pharyngeal wall and bilateral tonsils at the back of the uvula (palatine or uvula) with moderate force with a polyester fiber head swab, avoid touching the tongue, take out the posterior sampling tube, and break the plastic handle at the contact part of the hand, soak the swab into the sampling buffer, and tighten the cap. Throat swab samples can be preserved with a preservative solution such as normal saline or Hank's solution.
2. Precautions for sample collection: Avoid contamination during sample collection, storage and transfer.
Sample safety: All samples are considered to be potentially infectious, and operations are performed in accordance with relevant national standards.
3. Sample Storage
The samples to be tested must be stored frozen. The samples can be stored below -15°C for 1 week and can be stored for a long time below -70°C. The extraction of frozen samples should be performed after the samples are completely thawed, and repeated freezing and thawing should be avoided.
Suzhou Office: Building 1, No. 188, Fuchunjiang Road, High-tech Zone, Suzhou
Beijing Office: Building 2, No. 28, Yuhua Road, Airport Science and Technology Park, Shunyi District, Beijing
Contact number: +86-10-80497001
Nantong Office: No. 888 Zhujiang Road, Juegang Street, Rudong County, Jiangsu Province
Contact number: +86-513-80562882（Administration & Human Resources）