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Human Parainfluenza Virus III Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)

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Human Parainfluenza Virus III Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)

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Introduction

[Product Name]Human Parainfluenza Virus III Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)
[Packaging Size] 50 tests/kit
[Intended Use]

This kit is used for in vitro qualitative detection of parainfluenza virus type III nucleic acid in throat swab samples.
There are four types of human parainfluenza virus (HPIVs), which are common viruses that cause lower respiratory tract infection in children. Type III mainly causes pneumonia and bronchiolitis. HPIV III is prevalent in spring and summer, with the highest detection rate in summer. The incubation period of human parainfluenza virus is generally about 1 to 7 days.

The test results are not the only basis for the diagnosis of parainfluenza virus type III infection in patients. The clinical characteristics of the patient and other laboratory test data must be combined to correctly determine the infection or colonization of the pathogen, and formulate a reasonable treatment plan to make the treatment safe and effective.

[Test Principle]

The kit was to detect nucleic acid of HPIV III using Enzymatic Probe Isothermal Amplification (EPIA). Primers and Probe with RNA bases (rProbe) were developed tailored to the highly conservative sequences in genomes of HPIV III and added Bst enzyme and RNaseH. Two ends of RNA bases in rProbe were labeled with fluorophores as reporter and quencher. During amplification, the target sequence was amplified in isothermal condition using DNA polymerase and strand replacement of Bst enzyme, RNaseH cleaves RNA bases on target gene-probe hybrid strands, enabling to separate the reporter dye from the quencher dye and to generate the fluorescent signal. The remaining segment of rProbe RNA can continue the amplification process to generate fluorescent signals further. The fluorescent signals can be monitored by detection system, thereby realizing the detection of the target nucleic acid.

Recombinant pseudo-virus of Pine chloroplast gene was developed as internal control in the kit to monitor the quality in laboratory processing including validity of reagent and laboratory operation to avoid false negative.

[Storage Condition and Shelf-life]
The kit can be stored below -18°C and protected from light. The shelf life is 9 months. Repeated freezing and thawing cannot exceed 4 cycles. It can be transported stably for 5 days at low temperature protected from light.
See the packaging label for the production date, production batch number and expiration date.
[Applicable Instruments]

Applied Biosystems 7500 Real-Time PCR Systems, SLAN ®-96P Real-Time PCR Systems, LightCycler® 480 Real-Time PCR system, Easy Amp Real-time Fluorescence Isothermal Detection System(HWTS1600)
[Requirements for Samples]

1. Sample Collection
Freshly collected throat swabs.
Wipe the posterior pharyngeal wall and bilateral tonsils at the back of the uvula (palatine or uvula) with moderate force with a polyester fiber head swab, avoid touching the tongue, take out the posterior sampling tube, and break the plastic handle at the contact part of the hand, soak the swab into the sampling buffer, and tighten the cap. Throat swab samples can be preserved with a preservative solution such as normal saline or Hank' s solution.

2. Precautions for sample collection: Avoid contamination during sample collection, storage and transfer.
Sample safety: All samples are considered to be potentially infectious, and operations are performed in accordance with relevant national standards.

3. Sample Storage
The samples to be tested must be stored frozen. The samples can be stored below -18°C for a week and can be stored for a long time below -70°C. The extraction of frozen samples should be performed after the samples are completely thawed, and repeated freezing and thawing should be avoided.

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