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Borrelia Burgdorferi Nucleic Acid Detection Kit (Fluorescence PCR)

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Category:Infectious Diseases

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Borrelia Burgdorferi Nucleic Acid Detection Kit (Fluorescence PCR)

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Introduction

[Product Name] Borrelia Burgdorferi Nucleic Acid Detection Kit (Fluorescence PCR)

[Packaging Size] 20 tests/kit, 50 tests/kit
[Intended Use]
This product is suitable for in vitro qualitative detection of Borrelia burgdorferi nucleic acid in the whole blood of patients, and provides auxiliary means for the diagnosis of Borrelia burgdorferi patients. The test results are only for clinical reference, and the final diagnosis should be comprehensively considered in close combination with other clinical indicators. Lyme disease is caused by infection with Borrelia burgdorferi and is mainly transmitted between animal hosts, between host animals and humans by hard ticks. The pathogen Borrelia burgdorferi can cause human Erythema chronicum migrans, as well as diseases involving multiple systems such as heart, nerve, and joint, etc., and the clinical manifestations are diverse. According to the developing of diseases course, it can be divided into early localized infection, intermediate disseminated infection and late persistent infection, which are serious harm to the health of the population. Therefore, in the clinical diagnosis of Borrelia burgdorferi, it is of great significance to establish a simple, specific and rapid method for etiological diagnosis of Borrelia burgdorferi.

[Test Principles]

This kit uses the Taqman fluorescent probe method to design primers and probes of fluorescence detection for the highly conserved and specific regions of Borrelia burgdorferi. The FAM (Borrelia burgdorferi) fluorescent group and VIC (internal control) fluorescent group are labeled by two specific probes 5’ separately. During the detection and amplification process, specific primers and probes are combined with the target sequence. The formation of PCR products and the accumulation of fluorescent signals are completely synchronized by the DNA polymerase activity and 5’-3’ exonuclease activity of Taq enzyme, realizing the qualitative detection of Borrelia burgdorferi in the samples.

The kit contains human internal control, and the internal control probe labels VIC fluorescent group, the probe 3’ end labels quenching group. As a quality control for reagents, DNA quality, and the operation itself, avoid false negative test results.

[Storage Conditions and Shelf-life]

Storage condition: This kit should be stored below -18°C away from light, and the shelf-life is 12 months. The repeated freezing and thawing should be avoided (no more than 4 cycles). It should be frozen below -18°C for storage after opening, and if stored at 2~8°C, please use it within a week. Transportation condition: The kit placed in a transport box containing dry ices can be stably stored for 5 days.
See the label for the production date, production batch number and expiry date.

[Applicable Instruments]

Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Shanghai Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, and BioRad CFX Opus 96 Real-Time PCR System.

[Requirements for Samples]

1. Sample type: Whole blood sample.
2. Sample collection: Freshly collected EDTA anticoagulant blood.
3. Precautions for sample collection: Avoid contamination during sample collection, storage and transfer.
Sample safety: All samples are considered to be potentially infectious, and operations are performed in accordance with relevant national standards.
4. Storage
The collected samples should be sent for test as soon as possible. Samples that cannot be tested within 24 hours should be stored below -18°C for no more than 2 months, and stored below -70°C for long-term storage. The samples should be stored separately in a special warehouse or counter.

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