SARS-CoV-2 influenza A influenza B Nucleic Acid Combined Detection Kit (Fluorescence PCR)

Short Description:

This kit is suitable for in vitro qualitative detection of SARS-CoV-2, influenza A and influenza B nucleic acid of the nasopharyngeal swab and oropharyngeal swab samples which of the people who were suspected infection of SARS-CoV-2, influenza A and influenza B. 


Product Detail

Product Tags

Product name

HWTS-RT060A-SARS-CoV-2 influenza A influenza B Nucleic Acid Combined Detection Kit (Fluorescence PCR)

Epidemiology

Corona Virus Disease 2019 (COVID-19) is caused by SARS-CoV-2 which belongs to β Coronavirus of the genus. COVID-19 is an acute respiratory infectious disease, and the crowd is generally susceptible. At present, the SARS-CoV-2 infected patients are the main source of infection, and the asymptomatic patients may also become a source of infection. Based on the current epidemiological investigation, the incubation period is 1-14 days, mostly 3-7 days. The main manifestations were fever, dry cough and fatigue. A few patients have symptoms such as nasal congestion, runny nose, sore throat, myalgia and diarrhea.

Influenza is an acute respiratory infection caused by influenza virus. It is highly infectious and spreads mainly through coughing and sneezing. It usually breaks out in spring and winter. There are three types of Influenza, Influenza A (IFV A), Influenza B (IFV B) and Influenza C (IFV C), both of them belong to the ortomyxovirus family. Influenza A and B, which are single-stranded, segmental RNA viruses, are the main causes of human diseases. Influenza A is an acute respiratory infectious disease, including H1N1, H3N2 and other subtypes, is easy to change. the global outbreak, "shift" refers to the mutation of influenza A, resulting in a new viral "subtype". Influenza B is divided into two lineages: Yamagata and Victoria. Influenza B have only antigenic drift, and they evade surveillance and elimination by the human immune system through mutation. But influenza B viruses evolve more slowly than human influenza A, which also cause respiratory infections and epidemics in humans.

Channel

FAM

SARS-CoV-2

ROX

IFV B

CY5

IFV A

VIC(HEX)

Internal Control genes

Technical Parameters

Storage

Liquid: ≤-18℃ In dark  

Lyophilization: ≤30℃ in dark

Shelf-life

Liquid: 9 months

Lyophilization: 12 months

Specimen Type

Nasopharyngeal swabs, Oropharyngeal swabs

Ct

≤38

CV

≤5.0%

LoD

300 Copies/mL

Specificity

The cross test results showed that the kit was compatible with human coronavirus SARSr- CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, respiratory syncytial virus A and B, parainfluenza virus 1, 2 and 3, rhinovirusA, B and C, adenovirus 1, 2, 3, 4, 5, 7 and 55, human metapneumovirus, enterovirus A, B, C and D, human cytoplasmic pulmonary virus, EB virus, measles virus Human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata There was no cross reaction between Pneumocystis yersini and Cryptococcus neoformans.

Applicable Instruments:

It can match the mainstream fluorescent PCR instruments on the market.

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd. 


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